应用微透析技术测定天麻素对大鼠脑内乙酰胆碱释放量的影响  

Effect of gastrodin on release of extracellular acetylcholine in rat brain dialysate

作　　者：吕允凤[1] 

机构地区：[1]国家食品药品监督管理总局医疗器械技术审评中心,北京100044

出　　处：《药物分析杂志》2013年第10期1720-1725,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:考察天麻素对大鼠脑内乙酰胆碱释放的影响。方法:大鼠皮下给药后利用脑内微透析技术进行连续动态取样,建立高效液相色谱-串联四极杆质谱定量分析方法测定脑透析液中的乙酰胆碱含量,考察不同剂量给药前后大鼠脑内不同部位乙酰胆碱释放量的变化。结果:实验发现天麻素皮下给药能剂量相关地增加大鼠脑内不同脑区中乙酰胆碱的释放。结论:天麻素的脑内作用机制可能与其对胆碱能神经递质释放的调节作用有关。Objective： To study the effect of gastrodin on acetylcholine release in rat brain. Methods： A liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of acetylcholine in rat brain dialysates.The dialysates were prepared by microdialysis before and after subcutaneous administration of gastrodin at different dosages. Results： The results indicated that gastrodin significantly increased acetylcholine release in rat medial prefrontal cortex,nucleus accumbens and hippocampus in a dose-related manner. Conclusion： The mechanism of gastrodins action in the brain may be related to the regulation of cholinergic neurotransmitter release.

关 键 词：天麻素作用机制 乙酰胆碱 中层额前皮质 伏核 海马 脑微透析技术 液相色谱-串联质谱 

分 类 号：R917[医药卫生—药物分析学]