HPLC测定牛黄消炎片中的华蟾酥毒基和脂蟾毒配基的含量  

HPLC determination of cinobufagin and resibufogenin in Niuhuangxiaoyan tablets

作　　者：郑笑为[1] 张亚双[2] 汪祺[1] 刘燕[1] 戴忠[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]苏州市药品检验所,苏州215104

出　　处：《药物分析杂志》2013年第10期1774-1778,1786,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立牛黄消炎片中华蟾酥毒基和脂蟾毒配基2个成分的HPLC含量测定方法。方法:采用Agilent SB C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.5%磷酸二氢钾水溶液(用磷酸调pH至3.2)(37∶63),柱温为40℃,流速为1.0mL·min-1,检测波长为296 nm。结果:2个成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率分别为99.7%、99.8%。结论:该方法简便、准确、灵敏,可用于牛黄消炎片的质量标准提高研究。Objective： To establish an HPLC method for determination of cinobufagin and resibufogenin in Niuhuangxiaoyan tablets. Methods： The separation was performed on Agilent SB C18（4.6 mm×250 mm,5 μm）column.The mobile phase was composed of acetonitrile and 0.5% monopotassium phosphate solution（the pH adjusted to 3.2 by phosphoric acid）（37： 63）at a flow rate of 1.0 mL·min-1 and the detected wavelength was set at 296 nm,the column temperature was 40℃. Results： Both the two compounds showed good linearity in the range of the test concentration.The RSD of the precision,reproducibility and stability tests were all less than 3%,and the average recoveries were 99.7%,and 99.8% respectively. Conclusion： This method is simple,sensitive,and accurate,and it can be used for improvement of quality standard of Niuhuangxiaoyan tablets.

关 键 词：高效液相色谱 牛黄消炎片 华蟾酥毒基 脂蟾毒配基 蟾酥 含量测定 

分 类 号：R917[医药卫生—药物分析学]