胞内、外Ca^(2+)在水杨酸诱导丹参幼苗迷迭香酸生物合成过程中的作用  

Regulation effects of intracellular and extracellular Ca^(2+) on biosynthesis of rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza

作　　者：曹蓉蓉[1] 党小琳[1] 行冰玉[1] 张婧一[1] 董娟娥[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《中国中药杂志》2013年第20期3424-3431,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31170274);西北农林科技大学青年骨干支持计划项目

摘　　要：目的:为探讨胞内、外钙离子是否在水杨酸诱导丹参迷迭香酸生物合成中起作用。方法:以生长50 d的丹参幼苗为材料,通过喷施水杨酸诱导迷迭香酸合成量增加,再利用胞外钙通道抑制剂verapamil(Vp)和LaCl3、胞内钙调素拮抗剂trifluoperazine(TFP)和胞内钙通道上IP3受体抑制剂LiCl处理,分别考察水杨酸、钙离子通道抑制剂/钙调素拮抗剂处理后迷迭香酸的合成量及其合成相关酶(PAL,TAT)的活性变化。结果:2.0 mmol·L-1的水杨酸处理24 h后可诱导迷迭香酸合成量升高到(40.51±2.16)mg·g-1,是对照的1.97倍,迷迭香酸合成相关酶PAL活性升高到对照的1.42倍,TAT活性升高到对照的1.29倍;Vp,LaCl3,TFP,LiCl处理均抑制了水杨酸诱导的PAL,TAT活性的升高,从而导致了迷迭香酸合成量降低。结论:水杨酸诱导丹参迷迭香酸生物合成过程中,胞内、外钙离子发挥着重要的信号转导作用。Objective： To investigate the effect of intracellular and extracellular Ca2＋ on the biosynthesis of rosmarinic acid （RA） induced by salicylic acid in young seedlings of Salvia miltiorrhiza. Method： Young seedlings of S. miltiorrhiza were used to se- lect an optimal concentration of salicylic acid（ SA）, and then use the optimal concentration of SA to investigate the effects of extracellu- lar Ca2＋ channel inhibitors Verapamil, LaC13 , intracelluar calmodulin antagonist TFP and intracelluar Ca2＋ channel inhibitors LiC1 on the biosynthesis of RA and related enzymes. Result： SA increased the accumulation of RA and the activities of PAL and TAT, espe- cially the SA of 2 mmol ＋ L- 1 after 24 h. SA improved the accumulation of RA to （40.51 ± 2.16） mg · g- 1, which was 1.97 times than that of control, and the activities of PAL, TAT were 1.42 times and 1.29 times than those of the control. However, Vp, LaC13 , TFP, LiC1 inhibited the effects of SA evidently. Conclusion： Ca2＋ plays a key role in the regulation of the induction process.

关 键 词：丹参 水杨酸 PAL和TAT 迷迭香酸 钙通道抑制剂 钙调素拮抗剂 

分 类 号：S567.53[农业科学—中草药栽培]