农吉利碱脂质体中药物含量及包封率测定方法的研究  

Study on determination method for components in monocrotalinum liposomes and their entrapment efficiency

作　　者：熊阳[1] 章德军[1] 薛清芳 黄绳武[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053

出　　处：《中国中药杂志》2013年第20期3489-3492,共4页China Journal of Chinese Materia Medica

基　　金：浙江省科技厅项目(2012R10044-09);浙江省中医药管理局青年教师资助项目(2010ZQ001)

摘　　要：该文建立了HPLC测定农吉利碱脂质体中药物含量的方法，农吉利碱在1．6～102．4mg·L^-1线性关系良好（r=0．9998），日内精密度、日间精密度、稳定性、重复性的RSD分别为0．61％，0．92％，1．7％，1．6％，农吉利碱的回收率为（99．96±0．50）％，表明HPLC可以准确测定农吉利碱脂质体中药物；同时建立了微柱离心法测定农吉利碱脂质体中药物包封率，游离药物的柱回收率为（94．44±0．77）％，空白脂质体柱回收率为（95．86±0．68）％，平行测定3份农吉利碱脂质体的包封率，其RSD为4．0％，说明微柱离心法测定农吉利碱脂质体包封率方法准确可行。To establish an HPLC method for determining components in monocrotalinum liposomes. The results showed a good linear relationship in monocrotalinum liposomes within the concentration range between 1.6-102. 4 mg · L ^- 1 （ r = 0. 999 8 ）, with RSDs of intra-day precision, inter-day precision, stability and reproducibility of 0. 61% , 0. 92%, 1.7% , 1.6% , respectively. The recovery rate of monocrotaline was （99. 96 ±0. 50） %. These data indicated that the HPLC method could accurately determine components in monocrotalinum liposomes. Meanwhile, the microcolumn centrifugation method was established to determine the entrapment efficiency of components in monocrotalinum liposomes. As a result, the recovery rate and the b｜ank liposome recovery of free components were （94. 44 ±0. 77 ） %, （ 95.86 ±0. 68 ） %, respectively. According to the parallel determination of the entrapment efficiency of three monocrotaline liposomes, their RSD was 4. 0%. The data indicated that the microcolumn centrifugation method was an accurate and feasible method for determining the entrapment efficiency of monocrotaline liposomes.

关 键 词：农吉利碱 HPLC 包封率 微柱离心法 

分 类 号：R284[医药卫生—中药学]