热药吴茱萸对利血平所致虚寒证大鼠肝脏能量代谢相关机制的影响  

Effect of Euodiae Fructus on hepatic energy metabolism-related mechanisms of reserpine-induced asthenia cold syndrome rats

作　　者：黄丽萍[1] 余日跃[1] 彭淑红[1] 李森[1] 赵永佳[1] 刘红宁[1,2] 

机构地区：[1]江西中医药大学药学院,江西南昌330004 [2]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中国中药杂志》2013年第20期3539-3543,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2006CB504702);江西省自然科学基金项目(2010GZY0170;2010GZY0145);江西省教育厅项目(GJJ12519)

摘　　要：目的:探讨热药吴茱萸对虚寒证大鼠肝组织线粒体能量代谢相关机制的影响。方法:大鼠皮下注射利血平造模,经吴茱萸灌胃给药12 d后,氧电极法测定肝线粒体的呼吸效率;RT-PCR法测定肝组织Cox4,Atp5b,Ucp2,Pgc-1α,Nrf1,Tfam mRNA的表达量。结果:吴茱萸生药4.2 g·kg-1能显著升高虚寒证大鼠ST3及RCR,明显增加Cox4,Ucp2,Nrf1 mRNA表达量,亦有升高Atp5b,Pgc-1αmRNA表达量的趋势但无统计学意义,Tfam mRNA表达量变化不明显;吴茱萸生药2.1 g·kg-1能明显升高虚寒证大鼠ST3及RCR,显著降低P/O,明显升高Pgc-1αmRNA表达量和升高Nrf1 mRNA表达量的趋势,对Cox4,Atp5b,Ucp2,Tfam mRNA表达量无明显影响。结论:吴茱萸可能通过促进Pgc-1αmRNA和Nrf1 mRNA的表达,调节线粒体呼吸链中Cox4,Atp5b mRNA表达量,增加线粒体呼吸功能及氧化磷酸化效率。吴茱萸还可能通过增加Pgc-1α,激活肝脏中Ucp2 mRNA的表达使线粒体解耦联呼吸增强,产热增加。Objective： To discuss the effect of Euodiae Fructus on hepatic energy metabolism-related mechanisms of mitochon- dria of hepatic tissues of asthenia cold syndrome rats. Method： Rats were subcutaneously injected with Reserpine to establish the mod- el. After the oral administration with Euodiae Fructus for 12 d, the oxygen electrode method was adopted to determine the respiration efficiency. The expressions of Cox4, Atp5b, Ucp2 ,Pgc-1α, Nrfl, Tfam mRNA were assayed by using RT-PCR method. Result： Euo- diae Fructus 4. 2 g ·kg^-1 could obviously increase S33 and RCR of asthenia cold syndrome rats, and expressions of Cox4, Ucp2 Nrfl mRNA. It could also increase expressions of AtpSb and Pgc-1α mRNA, but with no statistical significance. No obvious change was ob- served in Tfam mRNA expression. Euodiae Fructus 4. 2 g·kg^-1 could significantly increase ST3 and RCR of asthenia cold syndrome rats and Pgc-lot mRNA and Nrfl mRNA expressions, and significantly decrease P/O, with no obvious impact on Cox4, AtpSb, Ucp2, Tfam mRNA expressions. Conclusion： Euodiae Fructus can promote mitochondrial respiratory function and oxidative phosphorylation ef- ficiency by improving Pgc-la mRNA and Nrfl mRNA expressions and regulating Cox4 and AtpSb mRNA in mitochondrial respiratory chain. It can also strengthen mitochondrial uncoupling respiration and add heat production by activating Ucp2 mRNA expression in liver.

关 键 词：吴茱萸 寒热药性 能量代谢 Uc p2 Cox4 Atp5b PGC-1Α Nrf1 

分 类 号：R285.5[医药卫生—中药学]