CQM对三叉神经痛模型大鼠的镇痛效应及其对脑内兴奋性氨基酸递质的影响  

Analgesic effect of CQM on prosopalgia model rats and its impact on exciting amino acid neurotransmitters

作　　者：王晔[1] 王丹巧[1] 崔悦[2] 张莹[1] 孙丹丹[1] 赵小亮[1] 刘洋[1] 张美玉[1] 焦玥[1] 徐晓军[3] 徐奭[4] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]吉林大学白求恩医学院,吉林长春130000 [3]卡罗林斯卡医学院,瑞典斯德哥尔摩14186 [4]首都师范大学生命科学学院,北京100048

出　　处：《中国中药杂志》2013年第20期3554-3559,共6页China Journal of Chinese Materia Medica

基　　金：中国中医科学院国际科技合作项目(2010DFA31890)

摘　　要：目的:观察自拟处方CQM对光化学诱导的三叉神经痛模型大鼠镇痛效应及其对脑内兴奋性递质谷氨酸(Glu)的影响。方法:将雄性SD大鼠随机分为假手术对照组和光化学诱导三叉神经痛手术组,后者再分为模型组、加巴喷丁组(100mg·kg-1),CQM低、高剂量组(35,70 mg·kg-1)。采用机械痛敏测试的方法评价大鼠的疼痛行为,以机械痛阈反映药效。脑内微透析方法采集大鼠纹状体细胞外液,高效液相-荧光法检测其中Glu水平。结果:与对照组相比,光化学诱导的三叉神经痛模型大鼠的机械痛敏感行为明显增强(P<0.01),脑内Glu浓度显著升高(P<0.05)。与模型组相比,各用药组机械痛阈均有上升,其中CQM高剂量组在3个时间点显著升高(P<0.05),最高达到(23±7.3)g,加巴喷丁组在2个时间点显著升高(P<0.05),最高达到(20.5±9.2)g。而反映大鼠脑内Glu浓度的指标各用药组均显著低于模型组(P<0.05)。结论:CQM能减轻光化学诱导的三叉神经痛模型大鼠的机械疼痛敏感行为,其镇痛效应可能与降低纹状体细胞外液中Glu水平有关。Objective： To observe the analgesic effect of CQM on photochemically-induced prosopalgia model rats, and discuss its impact on the exciting amino acid neurotransmitter-glutamate （Glu）. Method： Male SD rats were randomly divided into the sham operation group and the prosopalgia group. And the latter was subdivided into the model group, the gabapentin group （ 100 mg·kg^-1 ）, and the CQM low-dose （ 35 mg ·kg^-1 ） and CQM high-dose （70 mg ·kg^-1 ） groups. The mechanical allodynia test was adopt- ed to evaluate the pain behavior of rats, and reflect the efficacy with the mechanical withdrawal thresholds. The rat striatum extra-cellu- lar fluid was collected by brain micro-dialysis. The Glu level of samples was measured by high performance liquid chromatography-fluo- rescene detector （HPLC-FLD）. Result： Compared to the control group, the threshold of the mechanical allodynia of the IoN injury group was decreased significantly （P 〈 0. 05）, and the concentration of Glu was increased dramatically （P 〈 0. 05）. Compared to the model group, the mechanical allodynia of photochemically-induced prosopalgia model rats increased significantly （ P 〈 0. 01 ）, with a notable increase in brain Glu concentration （P 〈 0. 05）. Compared with the model group, all of mechanical withdrawal thresholds in- creased. Among them, the CQM high-dose group showed a remarkably growth at three time points （P 〈 0. 05）, with the maximum up to （23 ±7. 3 ） g. And the gabapentin group showed a remarkably growth at two time points （P 〈 0. 05 ）, with the maximum up to （20. 5 ±9. 2） g. All of the drug groups showed significantly lower Glu concentrations in rat brains than the model group （P 〈 0. 05 ）. Conclusion ： CQM can ease the mechanical allodynia of photoehemieally-induced prosopalgia model rats. Its analgesic effect may be re- lated to the decrease of Glu concentrations in striatum extra-cellular fluid.

关 键 词：CQM 光化学法 三叉神经损伤 脑内微透析 谷氨酸 

分 类 号：R285.5[医药卫生—中药学]