高效液相色谱法同时测定玉泉丸中的葛根素及甘草酸  

Simultaneous determination of puerarin and glycyrrhizic acid in Yuquan Pills by HPLC

作　　者：史培颖[1] 林逢林 陈芳美 钟新珠 叶贤清 何承光 姚宏[2] 

机构地区：[1]福建农林大学蜂学学院中药资源与蜂疗系,福建福州350002 [2]福建医科大学药学院,福建福州350004

出　　处：《中国医院药学杂志》2013年第20期1657-1659,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:81202987,81303298);福建省自然科学基金(编号:2012J01131、2012J05134);福建省教育厅科研基金项目(编号:JA11088);福建农林大学青年教师基金(编号:2011XJJ12)

摘　　要：目的：建立同时测定玉泉丸中葛根素及甘草酸的HPLC方法。方法：采用Ultimate XB-C18 （4.6 mm ×250 mm，5μm）色谱柱；以不同浓度的乙腈（B）-0.2%醋酸溶液（A）为流动相，检测波长254 nm，流速1.0 mL·min^-1，柱温30 ℃。结果：葛根素及甘草酸均在1.56～200 μg·mL^-1浓度范围内具有良好的线性关系（r〉 0.999），平均加样回收率分别为97.2%和99.0%， RSD均小于3% （n=6）。结论：该法简便、快速，重现性好，可为玉泉丸质量控制提供定量分析方法。OIlJECTIVE To establish the HPLC method for the simultaneous determination of puerarin and glycyrrhizic acid in Yuquan pills. METHODS Chromatographic experiment was carried out on an Ultimate XB-C18 column （250 mm × 4. 6 mm, 5/zm） with a gradient elution composed of aeetonitrile （B） and 0. 2% acetic acid （A）. The flow rate was 1.0 mL·min^-1 and the detection wavelength was 254 nrru The column temperature was set at 30 ＇C. RESULTS The calibration curve showed good linearity over the range of 1.56 - 200/,g·mL^-1 for both of the ingredients determined （r〉0. 999）. The average recoveries for puerarin and glycyrrhizic acid were 97. 2% and 98. 0%, respectively with the corresponding RSDs below 3G （n = 6）. CON- CLUSION This method is simple, rapid, and reproducible. It can be used as a quantitative analysis method for quality control of Yuquan pills.

关 键 词：玉泉丸 葛根素 甘草酸 含量测定 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]