苍术炮制前后挥发油的急性毒性实验  

Experimental study on the acute toxicity of volatile oil from Rhizoma Atractylodis before and after processing

作　　者：刘艳菊[1] 肖波[1] 季光琼[1] 许安安[1] 李鑫[1] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065

出　　处：《中国医院药学杂志》2013年第20期1670-1673,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81073051);湖北省自然基金重点项目(编号:2011CDA024);中国中医科学院中药研究所自主选题(编号:2011ZDXK-02)

摘　　要：目的：通过实验确定生苍术及麸炒苍术挥发油的半数致死量（LD50），分析炮制对苍术挥发油急性毒性的影响。 方法：以等比浓度法灌胃，采用改良寇氏法计算生苍术及麸炒苍术挥发油对小鼠的半数致死量（LD50）。结果：生苍术挥发油LD50为2454.71 mg﹒kg^-1，LD50的95%可信限为2123.24～2837.92 mg﹒kg^-1；麸炒苍术挥发油LD50为5248.07 mg﹒kg^-1, LD50的95%可信限为4677.35～5888.43 mg﹒kg^-1。结论： 生苍术挥发油为低毒；而麸炒苍术挥发油为实际无毒。OBJECFIVE To determine the lethal dose （LD50） of volatile oil from Rhizoma Atractylodis and bran fried Rhizo- ma Atractylodis in mice, and to analyze the impacts on acute toxicity of volatile oil from Rhizoma Atractylodis through process ing. METHODS With equal proportion consistency intragastric infusion method, the lethal dose （LD50） of volatile oil from Rhizoma Atractylodis and bran fried Rhizoma Atractylodis was calculated using modified Kou Shi method. RESULTS The LI~ value of the volatile oil from Rhizoma Atractylodis was 2 454. 71 mg/kg, LI50 95%confidence limit was 2 123.24-2 837. 92 mg.kg ^-1 ; the LD50,value of the volatile oil from bran fried Rhizoma Atractylodis was 5 248. 07 mg.kg ^-1, LD50 95% confidence limit was 4 677. 35-5 888.43 mg.kg^-1. CONCLUSION The volatile oil from Rhizoma Atractylodis rating is low toxicity; vol- atile oil from bran fried Rhizoma Atractylodis is actual non-toxic.

关 键 词：苍术 炮制 挥发油 急性毒性 

分 类 号：R28[医药卫生—中药学]