无肾功能损伤大鼠肾草酸钙结石模型的筛选  

Experimental renal calcium oxalate calculus models without severe renal damage in rats

作　　者：张圣平[1] 蒲小勇[2] 洪良庆[3] 梁辉[1] 王宏亮[1] 孙睿[1] 诸运青 

机构地区：[1]南方医科大学附属深圳市龙华新区人民医院泌尿外科,深圳518109 [2]广东省人民医院泌尿外科 [3]中山大学附属第三医院泌尿外科

出　　处：《中华实验外科杂志》2013年第10期2139-2141,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 用最小的肾脏功能损伤筛选制作短时、经济和成石效果好的大鼠肾草酸钙结石模型.方法 将雄性SD大鼠90只随机分为两组.实验1采用1.0％氯化铵和不同浓度乙二醇（EC,0.1％、0.2％、0.4％、0.8％）处理.实验2单用不同浓度EG（0.1％、0.2％、0.4％、0.8％）处理.7～21 d后处死大鼠,测体质量、血、尿肌酐和尿草酸盐含量.苏木素-伊红（HE）染色观察肾组织病理改变及肾成石.结果 实验1中,不同浓度EG＋ 1.0％氯化铵处理7d后,大鼠尿草酸盐排泄量均高于对照组（P＜0.05）.用0.4％或0.8％ EG＋ 1.0％氯化铵处理14d后,大鼠肌酐清除率均低于对照组（P＜0.01）；光镜观察,肾皮质、髓质和肾乳头等处出现草酸钙结晶体,肾组织炎性损伤加重.实验2中,用0.4％或0.8％ EG处理7d后,尿草酸盐排泄量高于对照组（P＜0.01）,但肌酐清除率变化差异无统计学意义（P＞0.05）.光镜观察,肾皮质及间质未出现明显水肿,可观察到少量结石结晶但无炎性细胞浸润.结论 单用EG处理大鼠可以在没有严重肾损伤的情况下构造短时、经济和成石效果好的大鼠肾草酸钙结石模型.Objective To screen a short-term,economical and satisfied model of renal calcium oxalate calculus in rats with minimal renal damage.Methods Ninety male Sprague-Dawley （SD） rats were randomly divided into two groups.In group 1,the rats were treated with 1.0％ ammonium chloride and different concentrations of ethylene glycol （0.1％,0.2％,0.4％ and 0.8％）.In group 2,the rats were treated with different concentrations of ethylene glycol （0.1％,0.2％,0.4％ and 0.8％）.The body mass change,the serum creatinine and urine creatinine,and urinary oxalate excretion were measured.Conventional HE dyeing was carried out for kidney tissues and pathological changes,and the status of kidney stone formation was further observed.Results In group 1 treated with 1.0％ ammonium chloride and 0.1％ ethylene glycol for 7 days,the urinary oxalate excretion days was significantly higher than in control group （P ＜0.05）.In group 1 treated with 0.4％ or 0.8％ ethylene glycol ＋ 1.0％ ammonium chloride for 14 days,the creatinine clearance was lower than in control group （P ＜ 0.01）.Light microscopy showed that calcium oxalate crystals formed in renal cortex,medulla and renal papilla,and inflammatory damage of renal tissue was aggravated.In group 2 treated with 0.4％ or 0.8％ ethylene glycol for 7 days,the urine oxalate excretion was higher than in control group （P ＜0.01）,however,there was no statistically significant difference in the creatinine clearance （P ＞ 0.05）.Light microscopy showed no obvious interstitial edema in renal cortex and a small amount of stone crystals were seen with no inflammatory cells infiltration.Conclusion Ethylene glycol alone is a quich,economic and good calcium oxalate crystallized method in rats without severe renal damage.

关 键 词：尿石病 草酸钙 乙二醇 模型 动物 

分 类 号：R691.4[医药卫生—泌尿科学]