H_2S位于H_2O_2下游介导葡萄抗霜霉病过程  

H_2S acts downstream of H_2O_2 in the regulation of grapevine Resistance to Plasmopara viticola

作　　者：王文杰[1] 车永梅[1] 郭秀萍[1] 侯丽霞[1] 刘新[1] 

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,青岛266109

出　　处：《植物病理学报》2013年第5期475-485,共11页Acta Phytopathologica Sinica

基　　金：农业部948项目(2006-G26);青岛市自然基金项目(10-3-4-5-5-jch和12-1-4-5-(12)-jch)

摘　　要：以霜霉病抗性葡萄品种左优红和感病品种霞多丽为材料,研究硫化氢(H2S)在葡萄应答霜霉病过程中的作用及其与过氧化氢(H2O2)的相互关系。结果表明,接种霜霉病菌后,抗性品种左优红和感病品种霞多丽叶片均出现H2S含量的猝发、H2S合成酶基因表达量增加、抗病相关蛋白多酚氧化酶(PPO)和β-1,3葡聚糖酶(Glu)活性显著升高现象;且H2S清除剂次牛磺酸(HT)可以显著抑制霜霉病菌诱导的PPO和Glu活性升高,并导致葡萄的感病率和病情指数显著升高;同时外源H2S可降低两个葡萄品种的感病率和病情指数。说明,H2S是参与葡萄抗霜霉病过程的新的信号物质。受霜霉病菌侵染后,葡萄叶片H2O2含量猝发早于H2S,并且H2O2清除剂抗坏血酸(AsA)可显著抑制霜霉病菌所诱导的H2S含量的上升,而H2S清除剂HT对霜霉病菌诱导的H2O2含量变化影响不显著,推测,H2S作用于H2O2的下游参与葡萄抵御霜霉病过程。By using two grape cultivars,the resistant Zuoyouhong and the susceptible Chardonnay to Plasmopara viticola,the role of hydrogen sulfide （H2S） in grape resistance to P.viticola and the relationship between hydrogen sulfide （H2S） and hydrogen peroxide （H2O2） were studied.The results showed that the P.viticola infection induced the H2S bursts,the increase of L-/D-CDes expression and the enhanced polyphenol oxidase （PPO） and glucanase （Glu） activities in the leaves of Zuoyouhong and Chardonnay.In contrast,the H2S scavenger hypotaurine （HT） depressed the P.viticola-induced PPO and Glu activities,and elevated colonization frequency and index of P.viticola in Zuoyouhong and Chardonnay.Meanwhile,the exogenous application of H2S decreased the colonization frequency and index of P.viticola in the two grape cultivars.These results indicated that H2S played a role in the regulation of grape resistance to P.viticola as a signal molecule.Application of the NADPH oxidase inhibitor dibenziodolium （DPI） and the cell wall peroxidase inhibitor salicylhydroxamic acid （SHAM） significantly suppressed the P.viticola-induced the increase of H2O2,it suggested that the NAD-PH oxidase and the cell wall peroxidase pathways were the important resource of H2O2.Based on the observations of the H2O2 burst appeared earlier than H2S in the leaves of grape infected by P.viticola,and the AsA depressed the P.viticola-induced H2S increase,and the HT had no significant effect on the H2O2,it was concluded that H2S acted functions downstream of H2O2 in mediating grape resistance to P.viticola.

关 键 词：葡萄霜霉病 H2S H2O2 抗病性 

分 类 号：S432.2[农业科学—植物病理学]