山莨菪碱抑制硝普钠所致牛心内膜内皮细胞凋亡  

The Inhibitory Effect of Anisodamine on SNP-induced Apoptosis in Bovine Endocardial Endothelial Cell

作　　者：刘梅冬[1] 尢家騄[1] 钟林[1] 

机构地区：[1]湖南医科大学病理生理教研室,湖南长沙410078

出　　处：《中国医师杂志》2000年第10期599-601,共3页Journal of Chinese Physician

摘　　要：培养的牛心内膜内皮细胞 (bovineendocardialendothelialcells ,BECEC) ,用 2mmol/ml硝普钠 (sodi umnitroprusside,SNP)损伤 2 4h ,出现典型的病理性凋亡。在 0 2mg/ml山莨菪碱加入培养基中 30min后 ,再加入SNP( 2mmol/ml) ,共同孵育 2 4h。发现山莨菪碱保护组与SNP损伤组比较 ,凋亡细胞减少 ;琼脂糖电泳未出现明显的“梯状”条带 ;DNA断裂百分率也降低。结果证明 :山莨菪碱能减轻SNP所致牛心内膜内皮细胞凋亡。The bovine endocardial endothelial cells(BECEC) were cultured and treated with sodium nitroprusside (SNP),BECEC showed typic pathologic apoptosis.Anisodamine(0 2mg/ml) pretreatment significantly attenuated SNP(2mmol/ml,24h)-induced apoptosis in BECEC.Compared with SNP group(n=8),the anisodamine group(n=8) showed less apoptosis cells,no specific DNA ladder pattern in agarose gel electrophoresis and less increase of percentage of DNA fragmentation.The results indicate that anisodamine significantly attenuated SNP-induced apoptosis in BECE.

关 键 词：细胞凋亡 内皮细胞 硝普钠 山莨菪碱 

分 类 号：R96[医药卫生—药理学]