分子标记辅助选择聚合Xa7、Xa21和cry1C＊基因改良杂交水稻金优207的白叶枯病和螟虫抗性  

Pyramiding Xa7,Xa21 and cry1C* for the Improvement of Bacterial Blight and Borer Resistance of Hybrid Rice Jinyou 207 by Molecular Marker-Assisted Selection

作　　者：闫成业[1] 刘艳[1] 牟同敏[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室/国家植物基因研究中心,湖北武汉430070

出　　处：《杂交水稻》2013年第5期52-59,共8页Hybrid Rice

基　　金：国家转基因生物新品种培育科技重大专项(2011ZX08001-001,2011ZX08001-004)

摘　　要：以抗白叶枯病的华恢20(携带Xa7和Xa21基因)和抗螟虫的T1c-19(携带cry1C*基因)为供体,分别与先恢207进行杂交、回交和复交,运用分子标记辅助选择技术,将白叶枯病抗性基因Xa7、Xa21和螟虫抗性基因cry1C*聚合到水稻恢复系先恢207中,育成了2个携带Xa7+Xa21+cry1C*基因的改良恢复系株系,编号为CY10038-1和CY10039-1。再与金23A配制了2个组合金23A/CY10038-1和金23A/CY10039-1。通过对改良品系及其杂交组合白叶枯病和螟虫抗性鉴定、CRY1C*蛋白表达量测定以及对产量、稻米品质、生育期和主要农艺性状的分析,结果表明,2个改良系及其杂交组合金23A/CY10038-1和金23A/CY10039-1均抗ZHE173、GD1358、PXO61、PXO99、FuJ、YN24和HeN11等7个白叶枯病菌株;尽管2个杂交组合CRY1C*蛋白表达量低于其改良系,但在全生育期不防治的栽培条件下不受螟虫危害;2个杂交组合单株产量比金优207均明显提高,而稻米品质和主要农艺性状与金优207相似,具有良好的应用前景。Xianhui 207,the male parent of widely-used hybrid rice Jinyou 207（Jin 23A /Xianhui 207）,was used as the recipient parent to cross with two resistance varieties,Huahui 20 carrying Xa7 and Xa21 genes and T1c-19 carrying cry1C^＊gene,as donor parents,to improve the bacterial blight（BB） and borer resistance of Xianhui 207.Two improved lines,designated as CY10038-1 and CY10039-1,carrying the genes of Xa7,Xa21 and cry1C^＊,were developed by hybridizing,backcrossing and multi-crossing with the method of molecular marker-assisted selection.Crossed with Jin 23A,two hybrids Jin 23A / CY10038-1 and Jin 23A / CY10039-1 were made.The resistances of bacterial blight,leaf folder and stem borer,the expression of CRY1C^＊protein and main agronomic traits including yield and grain quality were studied for the new improved lines and their derived hybrids.The results showed that two improved lines and their hybrids were resistant to seven Xoo strains,i.e.,ZHE173,GD1358,PXO61,PXO99,FuJ,YN24 and HeN11.Though the CRY1C^＊expression in two hybrids was lower than that in the improved lines,the hybrids were highly resistant to leaf folder and stem borer in field conditions.The testing results of main agronomic traits and grain quality showed that two hybrids were similar to Jinyou 207 except for their higher yield than that of Jinyou 207.Thus,these new improved lines would have a good prospect in future hybrid rice breeding.

关 键 词：杂交水稻 金优207 白叶枯病抗性 螟虫抗性 分子标记辅助选择 基因聚合育种 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]