稻瘟病菌RhoGAP基因表达特征及其生物信息学分析  

Expression and Bioinformatic Analysis of Rho GTPase Activating Proteins in Magnaporthe oryzae

作　　者：叶文雨[1] 胡芳辉[1] 连璧[1] 陈晓[1] 余文英[1] 鲁国东[1] 陈继圣[1] 王宗华[1] 

机构地区：[1]福建农林大学生命学院,福建福州350002

出　　处：《热带作物学报》2013年第10期1991-1997,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31030004;31300132)

摘　　要：Rho GAPs(GTPase activating proteins)是真核生物中Rho GTP酶的负调控因子之一,通过激活Rho GTP结合蛋白内在的GTP酶活性,使其加速水解成结合GDP的失活态。全基因组分析指出稻瘟病菌有8个Rho GAP成员(MoRhoGAP),但是其功能不清。本研究利用在线工具分析了其蛋白MoRhoGAP蛋白结构域及其定点突变后的三级结构,以及MoRhoGAP的互作蛋白网络,构建了系统发育树;利用基因芯片和公共数据库分析了在稻瘟病菌不同发育和侵染阶段Rho GAP的基因表达情况,为进一步研究稻瘟病菌RhoGAP的功能提供了理论依据。Rho GAPs（GTPase-activating proteins） are negative regulators of Rho GTPases in eukaryotic organisms.They stimulate the intrinsic Rho GTPase activity and enhance the GTP hydrolysis from the GTP-bound Rho proteins to inactive GDP-bound state,causing their inactivation.Genome annotation shows that the rice blast fungus Magnaporthe oryzae has 8 Rho GAP members,named hereafter as MoRhoGAP.But it is not clear yet of their functions.In this report,we analyzed their functional domains,tertiary structure with site-specific mutations,their interactive proteins and their phylogenetic relationships through bioinformatic methods.Furthermore,we investigated their gene expression profile at different growth and infection stage through DNA-array and public databases.Our study will facilitate the further functional characterization of these RhoGAP members in the fungus.

关 键 词：MoRhoGAP 生物信息学 基因表达 稻瘟病菌 

分 类 号：Q943.2[生物学—植物学] Q785