HPLC法测定尿液中拉莫三嗪和奥卡西平的浓度  

Determination of lamotrigine and oxcarbazepine content in human urine by HPLC

作　　者：朱雪萍[1] 李豪[2] 杜斌[2] 

机构地区：[1]郑州大学第五附属医院药剂科,郑州450052 [2]郑州大学口腔医学院药学院,郑州450000

出　　处：《中国临床药理学杂志》2013年第10期780-782,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的建立HPLC测定尿液中原形药物拉莫三嗪和奥卡西平浓度的方法。方法以甲醇为萃取剂,氯唑沙宗为内标,填充柱为Diamomsil-C18柱(200 mm×4.6 mm,5μm),保护柱为相同填料的短柱(8 mm×4 mm,5μm),流动相为甲醇-水-三乙胺(400∶594∶6),检测波长为260 nm,流速为1.0 mL·min-1,进样量为20μL,柱温为40℃。结果拉莫三嗪、奥卡西平和内标的保留时间分别为7.7,15.0和17.3 min。拉莫三嗪和奥卡西平的检测限均为2μg·mL-1,平均方法回收率为96.57%～101.21%,日内、日间RSD≤9.41%,平均提取回收率≥90.08%。结论所建立的尿样测定方法,样品预处理简单、易行、重现性好、快速,能够满足体内药物分析的需要。Objective To establish an method for the determination of unchanged drug lamotrigine and oxcarbazepine in human urine. Methods The extractant was methanol. The internal standard was chlorzoxazone, Packed column as diamomsil-C18（4. 6 mm ×200 mm,5 μm）, guard column as same material short column（8 mm ×4 mm,5μm） ,methanol -water - triethylamine were used as the mobile phase（400： 594： 6）and the detection wavelength was 260 nm. Velocity of flow was 1.0 mL ·min- 1, and injection volume was 20μL. The column temperature was 40℃. Results The peak time of lamotrigine oxcarbazepine and internal standard were 7.7,15.0,17.3 min, repectively. Linear range of lamotrigine and oxcarbazepine in human urine were 5 - 100 μg · mL-1. The limit of lamotrigine and oxcarbazepine were 2 μg · mL-1. Their average recovery were 96. 57% - 101.21%. The intra - day and inter - day RSD were lower than 9. 41%. The average extraction recovery were higher than 90. 08%. Conclusion The method is simple, good reproducibility, rapide, economic, accurate. It is suitable for determination of drug analysis.

关 键 词：高效液相色谱法 拉莫三嗪 奥卡西平 尿液 

分 类 号：R969.1[医药卫生—药理学] R971.6[医药卫生—药学]