SUMO在蛋白表达中的应用  

Application of SUMO Protein in Fusion Expression System

作　　者：汪小杰 毛若雨[1] 张勇[1] 滕达[1] 王秀敏[1] 王建华[1] 

机构地区：[1]中国农业科学院饲料研究所农业部饲料生物技术重点开放实验室,北京100081

出　　处：《生物技术通报》2013年第10期28-33,共6页Biotechnology Bulletin

基　　金：国家科技支撑计划课题(2013BAD10B02;2011BAD26B02);国家自然科学基金(31372346;31302004)

摘　　要：毒性蛋白表达具有很大的技术挑战性,其不仅对宿主菌有毒性,而且易于被宿主内源性蛋白酶降解。融合表达是实现毒性蛋白表达的有效策略。目前常用的融合标签有硫氧还蛋白、绿色荧光蛋白、麦芽糖结合蛋白、类固醇异构酶、N利用基质A及谷胱甘肽-S-转移酶等。而SUMO标签除具有传统融合标签的特性外,还具有分子小、促进折叠以及能被SUMO蛋白酶1专一性识别等优势,因此广受关注。综述SUMO标签、SUMO蛋白酶切割特性及其表达系统在异源蛋白融合表达中的应用。The expression of toxic protein is more challenging than common protein. Fusion expression strategy may be a good choice to reslove this problem. Traditional fusion tags including thioredoxin, green fluorescent protein, maltose binding protein, ketosteroid isomerase, NusA, glutathione-s-transferase have been widely used. Besides the characteristics of the traditional fusion tags above, SUMO protein has other advantages, such as small molecules, promoting folding and specific cleaved by SUMO protease 1. As a result, it has been widely used recently. This article depicted the characteristics of SUMO and SUMO pretease. Additonally, the application of SUMO fusion expression system was elaborated.

关 键 词：SUMO 融合表达 SUMO蛋白酶1 毒性蛋白 

分 类 号：Q78[生物学—分子生物学]