血浆/血清MicroRNAs定量分析的校正策略  

Normalisation Strategies for Plasma/Serum MicroRNAs Quantitative Analysis

作　　者：王雁 汤纳平 邱云良 南雅萍 马璟 

机构地区：[1]中国医药工业研究总院国家上海新药安全评价研究中心,上海201203

出　　处：《生物技术通报》2013年第10期52-57,共6页Biotechnology Bulletin

基　　金：国家"十二五"重大专项基金资助项目(2012ZX09302002;2012ZX09505-001-003);国家自然科学基金项目(81273603)

摘　　要：血液循环microRNAs表达量的变化与各种疾病及组织损伤的相关性研究备受关注。但细胞的污染,RNA提取和处理过程中的试验误差都会不同程度的影响microRNAs定量分析的结果。各种校正策略的使用可以消除这些影响因素。就目前使用的不同校正策略的优缺点进行分析,便于研究者正确选择血液循环microRNAs定量分析的校正方法,从而获得准确可靠的生物学意义上的变化。In recent years, the researchs on assossication between the circulating microRNAs from blood and various ctisease or tissue damage have attracted much attention. However, the imprecision of microRNAs concentrations may be caused by cellular contamination or variance attributable to extract and process RNA. Several strategies have been proposed for normalising these causes. In this review we discuss the relative advantages and disadvantages of different normalisation strategies and instruct researchers to choose a valid normalisation strategy that will enable the measurement of biologically meaningful results.

关 键 词：RT-QPCR 循环microRNAs 校正策略 内参基因 

分 类 号：Q461[生物学—生理学]