醛基片PCR芯片技术在转基因玉米品系检测中的应用研究  

Specific Detection of GM Maize Events Using Aldehyde PCR Chip

作　　者：张明哲 张晓峰 陈曦 吴姗 陈笑梅 周圆 

机构地区：[1]浙江省检验检疫科学技术研究院,杭州310016 [2]舟山出入境检验检疫局,舟山316000

出　　处：《生物技术通报》2013年第10期66-70,共5页Biotechnology Bulletin

基　　金：浙江省科技厅重大专项(2011C12023);国家质检总局科技计划项目(2011IK249);浙江省科技厅公益性项目(2011C22065);浙江出入境检验检疫局科研项目(ZK200958)

摘　　要：针对9种转基因玉米品系Bt11、TC1507、Bt176、MON810、MON863、GA21、NK603、Mon88017、MIR604进行醛基片PCR芯片高通量检测和条件优化。特异性和灵敏度试验表明,醛基片PCR芯片的特异性较好,能应用于转基因玉米品系鉴定。而该方法应用于转基因检测的灵敏度为5%,有待于后续试验进一步优化。In this study, 9 kinds of genetically modified maize events Btl 1, TC1507, Bt176, MON810, MON863, GA21, NK603, Mon88017, MIR604 were under high-throughput detection and condition optimization with aldehyde PCR chips. According to the specificity arid sensitivity experiments, it showed that the specificity of the aldehyde PCR chip is good enough to be applied in the identification of genetically modified maize events. But the sensitivity of this method was 5%, if it is applied to the transgenic detection. It calls for further optimization in the follow-up experiments

关 键 词：醛基片PCR芯片 转基因检测 品系鉴定 

分 类 号：S513[农业科学—作物学]