普通菜豆抗锈病基因SCAR标记鉴定  

Identification of Rust Resistant Genes Based on SCAR Markers in Common Bean(Phaseolus vulgaris L.)

作　　者：吴星波[1,2] 岳欢[1,2] 郝俊杰[2] 张晓艳[2] 李红卫[2] 王珍青[2] 吕享华[2] 

机构地区：[1]西南大学园艺园林学院,重庆400715 [2]青岛市农业科学研究院,青岛266100

出　　处：《生物技术通报》2013年第10期81-86,共6页Biotechnology Bulletin

基　　金：现代农业产业技术体系专项资金(CARS-09);农业部"引进国际先进农业科学技术"项目(2012Z-52;2011-G1(3)-08);青岛市农科院博士基金项目

摘　　要：利用10个普通菜豆抗锈病基因SCAR标记(SK14、SA14、SI19、SBC6、SAD12、SAE19、UR11-GT2、KB126、SF10和SBA8)引物组,对78份生产和研究中常用的普通菜豆资源进行了抗锈病分子标记鉴定。在这78份资源中,SA14和KB126标记引物扩增无特异性,SI19和SAD12无扩增带。其中,8份资源含有SK14标记,23份资源含有SBC6标记,28份资源含有SAE19标记,5份资源含有UR11-GT2标记,62份资源含有SF10标记,76份资源含有SBA8标记。含有2-5个标记的资源共67份。明确了78份参试菜豆资源所含的抗锈病基因类型,表明含有多个抗锈病基因的菜豆资源较为常见。Ten SCAR primer combinations （SK14, SA14, SI19, SBC6, SAD12, SAE19, URll-GT2, KB126, SF10 and SBA8） of common bean rust resistant genes were used to check the genome DNA of 78 common bean accessions. The SCAR primer pairs, SA14 and KB126, amplified bands without difference among all the tested accessions ; SI19 and SAD12 were not amplified target bands at all ; SK14 marker appeared in 8 accessions, SBC6 marker in 23 accessions, SAE19 marker in 28 accessions, UR11-GT2 marker in 5 accessions, SF10 marker in 62 accessions, and SBA8 marker in 76 accessions. 2-5 SCAR markers appeared in 65 accessions respectively. The types of rust resistant genes in each of the 78 accessions have been identified, and we fou.nd common that several rust resistant genes aggregated in one common bean accession.

关 键 词：普通菜豆 SCAR 菜豆锈病 抗病基因 分子标记 

分 类 号：S643.1[农业科学—蔬菜学]