改良Trizol法提取高质量蒙古沙冬青总RNA  

Extraction of High Quality Total RNA from Ammopiptanthus mongolicus with Improved Trizol Method

作　　者：陈静[1] 高飞[1] 周宜君[1] 张孜宸[1] 李章磊[1] 曹玉震 张至玮 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《生物技术通报》2013年第10期87-92,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31070361);中央高校基本科研业务费专项基金(1112KYQN31);中央民族大学"985工程"项目(MUC98504-14;MUC98507-08);国家大学生创新训练项目(GCCX2013110015)

摘　　要：以木本植物蒙古沙冬青的叶片和根为材料,采用改良Trizol法提取总RNA。结果表明,与传统Trizol法相比,改良Trizol法能有效去除提取过程中残留的异硫氰酸胍、β-巯基乙醇以及植物本身的糖、酚类物质,获得高质量总RNA。改良Trizol法提取总RNA技术已被成功应用于蒙古沙冬青转录组数据库的建立。该方法可为木本植物提取高质量RNA提供借鉴。Total RNA was extracted using an improved Trizol method, with the leaves and roots of xylophyta, Ammopiptanthus mongolicus （ Maxim ） Cheng f. The result showed that compared to traditional Trizol method, the improved Trizol method can effectively remove residual guanidinium isothiocyanate, beta-mercaptoethanol in extraction process, as well as the sugar and polyphenols of the plant itself, and high-quality total RNA was obtained. The improved Trizol method has been successfully applied to transcriptome database constrction of A. mongolicus. It also provided a reference for xylophyta to extract high-quality RNA.

关 键 词：总RNA提取 蒙古沙冬青 改良Trizol法 

分 类 号：S793.9[农业科学—林木遗传育种] Q943[农业科学—林学]