7号染色体46765080位点SNP与绵羊尾臀性状相关性研究  

The Correlation Between a Novel SNP on Chromosome 7 and the Trait of Sheep Tail or Rump

作　　者：梁耀伟[1] 张伟[1] 沈敏[1] 李欢[1] 高磊[1] 杨井泉[1] 刘守仁[1] 甘尚权[1] 王新华[1] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,石河子832000

出　　处：《生物技术通报》2013年第10期103-108,共6页Biotechnology Bulletin

基　　金："973"前期专项项目(2011CB111501);国家自然科学基金新疆联合基金重点项目(U1130302)

摘　　要：绵羊尾(臀)脂性状是重要耐逆性状,其脂肪沉积的分子机制不清。旨在研究绵羊脂尾(臀)沉积脂肪的分子遗传机制,以最近文献报道的7号染色体一处SNP位点为候选分子标记,利用PCR-RFLP方法检测该位点在尾型极端差异的阿勒泰羊、哈萨克羊、湖羊、中国美利奴细毛羊以及萨福克羊群体中的多态性,并采用模型分析其与尾(臀)脂性状的相关性。结果表明,7号染色体46765080位点的G等位基因高频出现在表型分值较高的臀脂型阿勒泰羊群体中,A等位基因在长瘦尾绵羊品种中高频出现;等位基因频率G/A的比值与尾臀表型分值相关性模型表明G/A比值随着尾臀表型分值增加呈指数增长。以上结果表明,绵羊7号染色体46765080位点在尾(臀)脂与瘦尾绵羊群体中分布存在显著性差异,该SNP位点可作为一个理想的分子标记应用于高、低脂绵羊品种选育。The fat-tailed or fat-rumped is one of important trait of stress tolerance, however, the mechanism of fat deposition is still unclear. Therefore, the present study choose a novel SNP reported in recently literature as a candidate molecular marker and detect the polymorphism among Altay, Kazakh, Hu, Chinese Merino and Suffolk which has extreme differences in tail type by using PCR-RFLP method. In addition, a mathematical model was employed to analyze the correlation between the polymorphism and the trait of fat-tailed or fat-rumped. Our results showed that the frequency of G allele was highest in Altay which had higher phenotype score, and the frequency of A allele was rather higher in thin-tailed sheep breeds. The result of mathematical model showed that the ratio of G/A increased exponentially with the phenotype score increasing. Together, the results suggested that there was significant differences in the SNP distribution between fat-tailed and thin-tailed sheep populations, and the SNP could be used as an idea/molecular marker to help high and low fat sheep breeding.

关 键 词：耐逆性状 脂尾性状 阿勒泰羊 多态性 基因型 

分 类 号：S826[农业科学—畜牧学]