不同温度下VC对小鼠卵母细胞排卵后老化的调控作用  

The Role of Vitamin C in Regulation of Aging of Postovulatory Mouse Oocytes Under Different Temperature

作　　者：李倩[1,2] 崔龙波[1] 赵振军[1] 

机构地区：[1]烟台大学生命科学学院,烟台264005 [2]山东农业大学动物科技学院,泰安271018

出　　处：《生物技术通报》2013年第10期113-119,共7页Biotechnology Bulletin

基　　金：山东省自然科学基金项目(ZR2011CQ003)

摘　　要：为研究抗氧化剂维生素C(Vitamin C,VC)对小鼠卵母细胞排卵后老化的调控作用,找到有效抑制卵母细胞老化的方法,通过孤雌激活和胚胎发育试验系统研究了VC在不同温度下对小鼠卵母细胞孤雌激活敏感性的抑制以及对胚胎发育能力的维持情况,初步筛选了有效抑制卵母细胞老化的方案;并通过体外受精发育试验和细胞免疫组化试验,分别研究了本筛选方案对卵母细胞受精及受精后胚胎发育的能力,以及对皮质颗粒分布和抗凋亡蛋白——BCL2蛋白水平的影响。结果显示,卵母细胞在添加200μmol/L VC的HCZB中于15℃培养24 h后,37℃恢复培养6 h是本试验筛选的能有效抑制小鼠卵母细胞排卵后老化的优化方案,说明一定浓度的抗氧化剂VC联合适当低温可以有效抑制卵母细胞的老化。To study the role of Vitamin C in regulation of aging of postovulatory mouse oocytes and find a suitable way to prevent oocytes aging, newly ovulated mouse oocytes were cultured at various temperature for various time in HCZB containing various concentrations of Vitamin C before examining for parthenogenesis activation and developmental potential. To assess the quality of oocytes cultured under optimization scheme that could effectively inhibited oocytes aging, early developmental competence after in vitro fertilization, distribution of cortical granule and levels of protein BCL2 were evaluated. Oocytes were cultured in HCZB with 200 μmol/L Vitamin C at 15℃ for 24 h before a 6 h recovery culture at 37℃ , which was the optimization scheme for inhibiting oocytes aging. The results suggested appropriate concentration of Vitamin C incorporating appropriate low temperature could effectively inhibit oocytes aging.

关 键 词：小鼠 卵母细胞 老化 VC 皮质颗粒 BCL2 

分 类 号：Q813[生物学—生物工程]