枯草芽孢杆菌BS24在苹果叶面的定殖及其对叶面菌群的影响  

Colonization of Bacillus subtilis BS24 on the Apple Leaf Surface and Their Effects on the Leaf Microbial Flora

作　　者：冉淦侨[1] 王楠[2] 戴佳锟[3] 赵文娟[1] 任平[1] 秦涛[1] 

机构地区：[1]陕西省科学院酶工程研究所,西安710600 [2]郑州轻工业学院食品与生物工程学院,郑州450001 [3]陕西省酶工程技术中心,西安710600

出　　处：《生物技术通报》2013年第10期131-136,共6页Biotechnology Bulletin

基　　金：陕西省科学院青年基金项目(2010k-27)

摘　　要：为了进一步明确枯草芽孢杆菌BS24对苹果早期落叶病的生防作用,分别对BS24菌株在苹果叶面上的定殖作用及其对叶面其它微生物种类和数量的影响进行了考察。结果表明,在田间条件下,BS24菌株能在苹果叶面成功定殖,但随时间的变化呈下降趋势;在无降雨等恶劣天气影响的情况下,喷施菌剂15 d后,叶面检测到的BS24菌株的活菌量从最初的2.52×107CFU/g成熟叶下降至2.11×105CFU/g成熟叶。与未喷施菌剂的叶面微生物菌群对比,喷施菌剂后苹果叶面菌群的数量显著下降;且种类上,喷施菌剂后的苹果叶面细菌种类少了栖稻假单胞菌、洛菲不动杆菌、Bacillus lehensis和玫瑰库克氏菌;真菌种类少了细交链孢菌、Eupenicillium janthinellum和大丽轮枝菌。To confirm the biocontrol efficacy of the Bacillus subtilis BS24 strain against apple early defoliation disease, the colonization of BS24 strains on apple leaves and their effects on the other microbial species and populations were studied respectively. The results showed that under field conditions BS24 strains could colonize on apple leaves, but the population size declined with prolonging time. In the absence of rain and other inclement weather situations, the population size of BS24 strain increased from the initial 2.5×107 cfu/g to 2.1×105cfu/g after spraying the microbial agents for 15 days. Compared with the apple leaf microorganisms without spraying agents, the microbial populations on apple leaves declined after 20 days of spraying agents, meanwhile the bacteria of Pseudomonas oryzihabitans, Acinetobacter lwoffii, Bacillus lehensis and Kocuria rosea and the fungi of the Alternaria alternate, Eupenicillium janthinellum and Verticillium dahliae were not detected.

关 键 词：苹果早期落叶病 枯草芽孢杆菌 定殖 微生物分离纯化 分子鉴定 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]