三氯杀螨醇与小牛胸腺DNA作用模式研究  

Study on the Binding Mode of Dicofol with Calf Thymus DNA

作　　者：李瑜[1] 张国文[1] 张业鹏[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《分析科学学报》2013年第5期610-614,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21167013);食品科学与技术国家重点实验室基金(No.SKLF-KF-201203;SKLF-ZZB-201305;SKLF-ZZA-201302)

摘　　要：在生理酸度pH=7.4条件下,运用荧光光谱、圆二色谱(CD)和傅里叶变换红外光谱(FT-IR)等多种光谱技术,并结合熔点和粘度测量,研究了三氯杀螨醇与小牛胸腺DNA的相互作用模式。实验发现,三氯杀螨醇使DNA的熔点和粘度增大,DNA-溴化乙锭(DNA-EB)复合物的荧光显著猝灭,表明三氯杀螨醇与DNA发生嵌插结合。FT-IR和CD分析推测三氯杀螨醇主要与鸟嘌呤和胞嘧啶碱基结合,并诱导DNA的双螺旋结构和碱基堆积发生一定程度的变化。The interaction between dicofol and calf thymus DNA was investigated in physiological buffer （pH 7.4） by fluorescence, circular dichroism（CD）, and fourier transform infrared（FT-IR） spectroscopy coupled with DNA melting study and viscosity measurements. It can be found that dicofol could intercalate into the base pairs of ctDNA as evidenced by significant fluorescence quenching of the ctDNA- ethidium bromide（EB） complex,and increasing of melting temperature and relative viscosity of ctDNA. Analysis of the FT-IR and CD spectra suggested that dicofol mainly bound to guanine and cytosine and led to some changes in base stacking and double helix of ctDNA.

关 键 词：三氯杀螨醇 小牛胸腺DNA 作用模式 光谱法 

分 类 号：O657.3[理学—分析化学]