尿miRNA-192,29c表达与芪蛭肾综颗粒治疗难治性肾病综合征机制研究  

Correlation of Urinary miRNA-192,29c Expression with Therapeutic Mechanism of Qizhi Shenzong Granula for Treatment of Refractory Nephrotic Syndrome

作　　者：张春花[1,2] 王明[1] 耿振波[1] 魏连波[1] 于淑军 

机构地区：[1]南方医科大学珠江医院,广东广州510282 [2]威海市中医院肾病科,山东威海264200

出　　处：《新中医》2013年第11期40-42,共3页New Chinese Medicine

基　　金：广东省科技技术厅与中医科学院联合科研专项项目(编号:2011B03220001)

摘　　要：目的:探讨尿miRNA-192、miRNA-29c在芪蛭肾综颗粒治疗难治性肾病综合征(RNS)的机制。方法:将RNS患者44例随机分为实验组(芪蛭肾综颗粒加基础治疗)23例及对照组(黄葵胶囊加基础治疗)21例,经3月治疗后,观察尿miRNA-192、miRNA-29c与24h尿蛋白定量、总胆固醇(TC)、载脂蛋白(α)[LP(α)]、白蛋白(Alb)等指标的相关性,治疗前后尿miRNA-192、miRNA-29c的变化。结果:2组总有效率比较,差异有显著性意义(P<0.05)。尿miRNA-29c与LP(α)呈负相关,与Alb呈正相关,差异均有非常显著性意义(P<0.01)。尿miRNA-192差值与24h尿蛋白定量和TC呈正相关,差异有显著性或非常显著性意义(P<0.05,P<0.01)。2组治疗后与治疗前比较,miRNA-29c升高,miRNA-192降低,差异有显著性或非常显著性意义(P<0.01,P<0.05);治疗后2组miRNA-29c差值比较,差异有显著性意义(P<0.05)。结论:芪蛭肾综颗粒能抑制RNS的细胞外基质(ECM)增生,延缓肾小球硬化。尿miRNA-192、miRNA-29c可能为新型的非侵入型的检验指标。Objective： To study the relationship of urine miRNA-192 and miRNA-29c expression with the therapeutic mechanism of Qizhi Shenzong Granula for the treatment of refractory nephrotic syndrome（RNS）. Methods： Forty-four RNS patients were divided into the test group and the control group, treatment lasting for 3 months. Both groups were given basic routine treatment, and additionally, the test group was given Qizhi Shenzong Granula and the control group was given Huangkui Capsules. The urine miRNA-192 and miRNA-29c expression levels were monitored before and after treatment, and their correlation with 24-hour urinary protein, total cholesterol（TC）, apoprotein cx [LP（e）], albumin（AIb） was investigated. Results： The expression of urinary miRNA-29c was positively correlated with AIb and negatively correlated with LP（α）（P〈 0.01）. The expression of urinary miRNA-192 was positively correlated with urinary 24-h urinary protein and TC（P〈 0.05, P〈 0.01）. With comparison to those before treatment, miRNA-29c expression was increased, miRNA-192 was decreased in the two groups after the treatment, the differences being significant（P〈 0.01, P〈 0.05）. The difference of miRNA-29c expression level was significant between the two groups after treatment （P 〈 0.05）. Conclusion： Qizhi Shenzong Granula has an effect on inhibiting the proliferation of extracellular matrix（ECM） and delaying the glomerular sclerosis. Urinary miRNA-192 and miRNA-29c can be expected as new non-invasive test indexes for refractory nephrotic syndrome.

关 键 词：难治性肾病综合征(RNS) 芪蛭肾综颗粒 miRNA-192 miRNA-29c 

分 类 号：R692[医药卫生—泌尿科学]