不同锌源对肉仔鸡肠道形态及金属硫蛋白表达的影响  

Effects of different-source zinc on intestinal structure and metallothionein mRNA level in broiler chicks

作　　者：郑立鑫[1] 刘华伟[1] 吴鹏华[1] 刘大森[2] 仝泳 

机构地区：[1]东北农业大学动物科技学院 [2]东北农业大学理学院 [3]东北农业大学生命学院

出　　处：《中国饲料》2013年第20期7-10,共4页China Feed

基　　金：黑龙江省研究生创新科研项目立项(YJSCX2012-025HLJ);高等学校博士学科点专项科研基金(20102325110005)

摘　　要：为研究同一添加水平的4种锌源对AA肉仔鸡小肠形态及金属硫蛋白(MT)表达的影响,选取1日龄AA肉仔鸡480只,随机分成4组,每组6个重复,每个重复20只鸡。在玉米-豆粕型基础饲粮(含锌22.68 mg/kg)中分别添加硫酸锌(ZnSO4)、赖氨酸锌(Lys-Zn)、蛋氨酸锌(Met-Zn)、甘氨酸锌(Gly-Zn),配制成4种含锌量均为90 mg/kg的试验饲粮,ZnSO4组为对照组,试验期21 d。结果表明:4种锌源对肉仔鸡小肠形态和肝脏MT含量均无显著影响(P>0.05)。Gly-Zn和Met-Zn组小肠MT mRNA相对表达量和胰脏MT含量显著高于对照组(P<0.05),但Lys-Zn组与对照组差异不显著(P>0.05)。在3种氨基酸螯合锌中,Lys-Zn组胰脏MT含量、十二指肠MT mRNA相对表达量显著低于其他两种螯合锌组(P<0.05)。由此可见,Gly-Zn和Met-Zn对促进肉仔鸡小肠MT mRNA表达,提高胰脏MT含量效果较好。The experiment was conducted to investigate the effects of four different-source zinc with the same levels on intestinal structure and metallothionein （MT） expression of broiler chicks.A total of 480 day-old male broiler chicks were assigned randomly into 4 dietary treatments with 6 replicate of 20 each.Dietary treatments included a basal corn- soybean meal diet supplemented with the ZnSO4,Lys-Zn,Met-Zn and Gly-Zn to ensure the zinc levels of the meal diet to 90 mg Zn/kg.The results showed as follow：There were no significant effect in intestinal structure and MT concentration in liver among four different-source zinc.MT concentration in pancreas and the MT mRNA levels in intestine of Met-Zn and Gly-Zn treated groups were significantly higher than those of inorganic control group （P 〈 0.05）,while Lys-Zn treated group had no significant effect compared with control group （P 〉 0.05）.MT concentration in pancreas and the MT mRNA levels in duodenum of Lys-Zn treated groups were significantly lower than those of Met-Zn and Gly-Zn group（P 〈 0.05）. Therefore, Met-Zn and Gly-Zn could increased the expression levels of MT mRNA in intestine and the pancreas MT con- centration in broiler chicks.

关 键 词：肉仔鸡 氨基酸螯合锌 表达 金属硫蛋白 小肠形态 

分 类 号：S816.7[农业科学—饲料科学]