动脉粥样硬化家兔红细胞L-精氨酸一氧化氮通路的变化  

Alteration of L-Arginine/Nitric oxide pathway in red blood cell of rabbit with atherosclerosis

作　　者：汪波[1] 姚兴海[1] 张宝娓[1] 张钧华[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院,100034

出　　处：《中国介入心脏病学杂志》2000年第4期210-212,共3页Chinese Journal of Interventional Cardiology

摘　　要：目的　观察动脉粥样硬化家兔红细胞L 精氨酸 (L Arg)转运及一氧化氮合酶 (NOS)活性的变化 ,探讨动脉粥样硬化发病机制。方法　选家兔 12只 ,分为两组 ,每组 6只 ,分别喂以高脂饮食(高脂组 )及普通饮食 (对照组 ) 6周 ,取静脉血浆测血脂水平。并测定红细胞的L Arg转运及NOS活性。结果　 (1)高脂组动物血浆总胆固醇 (Total Chol)、甘油三脂 (TG)及低密度脂蛋白胆固醇 (LDL C)水平明显高于对照组 (P <0 0 1) ;(2 )高脂组动物红细胞L Arg最大转运速率 (Vmax)较对照组下降19 1% (P <0 0 1) ,NOS活性较对照组降低 42 4% (P <0 0 1)。结论　动脉粥样硬化时红细胞L 精氨酸的跨膜转运障碍及一氧化氮合酶活性降低。Objective The changes of L Arginine transport and Nitric Oxide synthesis activity of Red blood cell (RBC) in atherosclerotic rabbits were observed in order to explore the mechanism of atherosclerotic development Methods 12 rabbits were divided into two groups (Hyperlipid group and Control group), each group containing 6 rabbits, Hyperlipid group was fed with high fat diet and control group fed with regular diet for 6 weeks Intravenous blood samples were detected on plasma lipid and L Arginine transport and NOS activities in RBC Results (1) plasmal Total Cholesterol、TG and LDL c levels of Hyperlipid group was higher than that of Control group ( P <0 01) respectively (2) The maximum velocity of L Arginine transport and NOS activities of RBC in Hyperlipid group decreased by 19 1% ( P <0 01) and 42 4% ( P <0 01), respectively compared with control group Conclusion Atherosclerosis could result in the abnormality of L Arginine transport and diminish activies NOS activity in RBC

关 键 词：L-精氨酸 一氧化氮合酶 动脉粥样硬化 红细胞 

分 类 号：R543.502[医药卫生—心血管疾病]