胰岛素样生长因子结合蛋白3和胰岛素样生长因子结合蛋白5基因在内蒙古白绒山羊皮肤中的表达研究  

Study on Expression of Insulin-like Growth Factor-binding Protein-3and -5(IGFBP-3 and IGFBP-5)in the Skin of Inner Mongolia Cashmere Goat

作　　者：于新蕾[1] 蔡婷[1] 俎红丽[1] 杨惠茹[1] 肖红梅[1] 刘志红[1] 王全喜[2] 王志新[1] 李金泉[1] 

机构地区：[1]内蒙古农业大学动物科学学院,动物遗传育种与繁殖重点实验室,内蒙古呼和浩特010018 [2]内蒙古农业大学研究生院,内蒙古呼和浩特010018

出　　处：《中国畜牧兽医》2013年第10期1-5,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家863计划(2013AA102506);国家自然科学基金(31072005;31272421);国家农业部现代农业产业技术体系(CARS-40-05);内蒙古自治区自然科学基金(2013MS0411);教育部博士点专项基金(2012151520009)

摘　　要：本研究旨在探明胰岛素样生长因子结合蛋白(insulin-like growth factor-binding protein,IGFBP)在内蒙古白绒山羊绒毛发育的不同生长阶段皮肤组织中的表达变化情况。试验采用实时荧光定量PCR技术,对内蒙古白绒山羊绒毛生长发育的各个时期皮肤中IGFBP-1～IGFBP-6基因的表达进行了测定,并通过埋植褪黑素组改变绒毛生长周期的研究,来鉴定IGFBP-1～IGFBP-6基因中高表达的结合蛋白的作用。结果表明,IGFBP-3和IGFBP-5在绒山羊绒毛生长发育的各个时期的皮肤组织中均有表达,但是IGFBP-5的表达相对于IGFBP-3的表达具有很强的规律性。结果提示,在内蒙古白绒山羊的绒毛发育过程中,IGFBP-5可能是一个主要的调控因子。This experiment was conducted to study the expression effect of insulin-like growth factor-binding protein（IGFBP）of Inner Mongolia Cashmere goat skin in different developmental stage.The expression of IGFBP-1 to IGFBP-6 in different developmental stage of hair follicle cycle was measured by Real-time PCR.Furthermore,the hair growth cycle was changed by implanting melatonin to identify the role of IGFBP-1 to IGFBP-6 gene,the high expression of the binding protein.The results showed that IGFBP-3 and IGFBP-5 both were expressed in different developmental stage of hair follicle cycle,however,the expression of IGFBP-5 gene presented more regular.The results indicated that IGFBP-5 might be a major regulatory factor in the process of hair follicle development of Inner Mongolia Cashmere goat.

关 键 词：内蒙古白绒山羊 IGFBP-3 IGFBP-5 毛囊周期 

分 类 号：Q78[生物学—分子生物学]