海南黑山羊小分子热休克蛋白9cDNA克隆及其生物信息学分析  

Cloning and Bioinformatics Prediction of Small Heat Shock Protein cDNA in Hainan Black Goat

作　　者：施力光[1] 曹婷[1] 侯冠彧[1] 周汉林[1] 黄显洲[1] 荀文娟[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《中国畜牧兽医》2013年第10期56-60,共5页China Animal Husbandry & Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国热带农业科学院热带作物品种资源研究所)(1630032012022)

摘　　要：本研究以海南黑山羊睾丸组织为材料,利用RT-PCR和RACE技术获得海南黑山羊小分子热休克蛋白9(small heat shock protein 9,Hspb9)基因完整的CDS序列,并对其进行生物信息学分析。结果表明,克隆得到山羊Hspb9cDNA长549bp,CDS区471bp,共编码157个氨基酸,提交至GenBank数据库中,登录号为:JX088726。生物信息学分析结果表明,Hspb9编码的蛋白无跨膜区和信号肽,存在多个磷酸化位点;预测Hspb9蛋白二级结构α-螺旋占15.28%,β-折叠占30.57%,无规则卷曲占54.15%;存在1个UBQ结构域;Clustel W方法比对山羊Hspb9与绵羊、牛的同源性最高。RACE and RT-PCR were used to clone the full length of small heat shock protein 9 coding sequence from Hainan Black goat testis and the bioinformatics analysis was performed.The results showed that the full length of Hspb9 cDNA was 549bp,including 471bp in CDS which encoded a protein of 157 amino acids.The sequence was submitted to GenBank,Accession No.was JX088726.It had no trans-membrane region and signal peptide of the Hspb9 protein.There were several phosphpryiation sites in the sequence.The protein contained 15.28% of α-helix,30.57% of β-sheet, and 54.15% of coil.An ubiquitin domain（UBQ） was also predicted.The Clustel W alignment results showed that goat Hspb9 had a close relationship with sheep and cattle.

关 键 词：小分子热休克蛋白9 海南黑山羊 CDNA克隆 生物信息学分析 

分 类 号：Q785[生物学—分子生物学]