鸭源新城疫病毒TH-1株的分离鉴定及遗传进化分析  

Isolation,Identification and Genetic Evolution Analysis of FGene of Newcastle Disease Virus TH-1 from Duck

作　　者：达娃卓玛[1] 金红岩[1] 封家旺[1] 杨康林[1] 姜一曈[2] 吕健[3] 侯绍华[2] 

机构地区：[1]西藏职业技术学院,西藏拉萨850030 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]国家知识产权局专利审查协作北京中心,北京100193

出　　处：《中国畜牧兽医》2013年第10期206-210,共5页China Animal Husbandry & Veterinary Medicine

基　　金：农业部动物疫情监测与防治项目(2013)

摘　　要：试验从吉林省通化某地发病死亡鸭子病料中分离到1株具有血凝性的病毒,通过血凝试验、血凝抑制试验、血清中和试验及融合蛋白(F)基因的扩增测序,初步鉴定为新城疫病毒,命名为TH-1株。该病毒鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)及6周龄鸡静脉接种致病指数(IVPI)分别为53.4h、1.85和2.575,表明该新城疫病毒为强毒。F蛋白多肽裂解位点为112-RRQKRF-117,符合新城疫强毒株裂解位点氨基酸序列,进一步证明该毒株为新城疫强毒株。F基因核苷酸及氨基酸同源性比对发现,TH-1株与中国野鸭源新城疫病毒分离株mallard China HLJ株的同源性最高,核苷酸同源性达到了99.3%,同为新城疫基因Ⅶ型毒株,与目前新城疫流行的主要基因型Ⅶ型相一致,为鸭源新城疫的有效防制提供了理论基础。A Newcastle disease virus（NDV）was isolated from the death duck in Jilin province Tonghua city, and TH-1 strain was named.It was identified with hemagglutination test,hemagglutination inhibition test,neutralization test,PCR and sequence analysis.It was proved virulent because of MDT of 53.4h,1.85 of ICPI, and 2.575 of IVPI.The cleavage site of the virus F protein was 112-RRQKRF-117,according to highly pathogenic strain character.The homology of Fgene was 99.3% with that of strain mallard China HLJ,separated from wild duck.By analyzing genotype and homology of TH-1 and other NDV strains,it showed that TH-1 strain was belonged to genotypeⅦ,which was the dominated genetype in China in recently.

关 键 词：鸭源新城疫病毒 分离 鉴定 融合蛋白基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]