用于多孔板细胞分析的自动显微成像系统  

Auto-microimaging system for cell analysis with multiple well plates

作　　者：韩西达 王敏[1] 黄强[1] 何永红[1] 周超英[2] 

机构地区：[1]清华大学深圳研究生院光学检测与成像实验室,广东深圳518055 [2]哈尔滨工业大学深圳研究生院机电工程与自动化学院,广东深圳518055

出　　处：《光学精密工程》2013年第10期2543-2548,共6页Optics and Precision Engineering

基　　金：国家自然科学基金资助项目(No.61275188,No.81171375);深圳市杰出青年基金资助项目;国家重点实验室开放基金资助项目(No.12K05ESPCT)

摘　　要：设计了用于多孔板细胞分析的自动显微系统以实现药物筛选的自动检测。研究了系统的机械结构、人机交互软件、对焦评价函数的选择和多视野图像拼接功能。该系统采用支架式结构,克服了形变带来的精度影响;对比传统机械传动的优缺点后,采用伺服电机和音圈电机两级调节的方式来提高系统精度和对焦速度。比较了几种对焦评价函数的性能并提出性能更优的评价方法。考虑到高倍显微镜下视野小对实验统计意义的影响,设计了多视野图像拼接等图像处理功能。最后,实验验证了系统的设计技术指标。实验结果表明:单次对焦时间为2s;对焦精度在微米级;单次4×4图像阵列拼接时间为15s,满足系统进行药物筛选对速度、自动化程度和稳定可靠性的要求。An auto-microimaging system to provide 3D scanning and auto-focus at each dimension was designed for the high throughput micro-imaging of drug screening.The mechanical structure,manmachine interaction software,the section of focusing evaluation function and the image titching of multi-view image for the system were researched.A rack structure was designed to overcome the limitation of accuracy from the deformation and a servo motor and a voice coil motor were combined as a two-stage controller to improve the accuracy and focus speed of the measuring system.Then,an algorithm of auto focus was presented after several auto-f-ocus evaluating methods were compared.According to the effect of a small view under the high power microscope on the experimental statistics,the function of image stitching was designed to fit the statistical significance of the experiment.On the experimental verification,it indicates that the time to complete the process of auto-focus is 2 s,and the accuracy of auto-focus can reach several micrometers.Moreover,the time to combine the microimaging regions of a 4 × 4 array is 15 s.The system can satisfy the requirements of short detection time,robotization,stability and reliability of cell analyzing with multiple well plates in drug screening.

关 键 词：显微成像 细胞分析 多孔板 自动对焦 图像拼接 

分 类 号：TH742.9[机械工程—光学工程] TH703[机械工程—仪器科学与技术]