一株高浓度烟碱降解菌的筛选、分离和初步鉴定  

Screening, Isolation and Identification of a High Concentration Nicotine Degradation Bacterium Strain

作　　者：龙章德[1] 韦方立[2] 李俊芳[3] 田兆福[1] 张敏[3] 黎新钦 申佩弘[3] 

机构地区：[1]广西中烟工业有限责任公司,南宁530001 [2]广西民族师范学院化学与生物工程系,崇左532200 [3]广西大学生命科学与技术学院,南宁530004

出　　处：《基因组学与应用生物学》2013年第5期612-615,共4页Genomics and Applied Biology

基　　金：广西中烟工业有限责任公司创新项目(20100037)资助

摘　　要：采用以烟碱为唯一碳源氮源的选择培养基,从烟草和植烟土壤中分离筛选出15株高浓度烟碱降解的菌株,其中菌株D9烟碱降解能力最强,其烟碱降解率为82%,耐受烟碱的最高浓度为8 g/L。经常规形态特征、16S rDNA同源序列以及系统进化树分析,初步鉴定该菌株为类似氧化微杆菌,命名为Microbacterium sp.GYC29。本研究为微生物降解烟碱的研究及应用提供了菌种资源。15 nicotine-degrading bacterial strains were separated and screened from tobacco and soil with selectivity culture medium, strain D9 was the best one with the highest nicotine degradation ratio reached at 82%, and could grow in a high nicotine concentration reached at 8 g/L using nicotine as the sole carbon and nitrogen source. The strain D9 was identified as Microbacterium sp. GYC29 according to the normal morphology, physiological and bio- chemical characters and 16S rDNA gene sequence.

关 键 词：烟碱 生物降解 烟叶 微杆菌 

分 类 号：S572[农业科学—烟草工业]