粉菠萝FVE同源基因的克隆及表达分析  

Cloning and Expression Analysis of FVE Homologous Gene from Aechmea fasciata

作　　者：李丽[1] 罗轩 徐立[1] 李新国[2] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室,海南省热带作物种质资源遗传改良与创新重点实验室,儋州571737 [2]热带作物种质资源保护与开发利用教育部重点实验室,海南大学园艺园林学院,海口570228

出　　处：《基因组学与应用生物学》2013年第5期621-626,共6页Genomics and Applied Biology

基　　金：海南省自然基金(808191);农业部热带作物种质资源利用重点开放实验室开放基金项目(KFKT-2011-06)共同资助

摘　　要：FVE是植物自主开花途径中的关键基因,主要通过抑制FLC的表达来调控花发育过程。本研究利用粉菠萝(Aechmea fasciata)茎尖组织转录组序列数据,通过RACE技术克隆获得了粉菠萝中FVE同源基因AfFVE的cDNA全长序列。该序列全长为1 672 bp,开放阅读框为1 404 bp,编码由468个氨基酸残基组成的蛋白,该蛋白序列中含有6个保守的WD重复区域。氨基酸序列比对结果表明,AfFVE基因编码的氨基酸序列与其它物种中FVE的同源序列具有较高的相似性。qRT-PCR(实时荧光定量PCR)分析结果显示,粉菠萝中AfFVE基因对外源乙烯的刺激产生了应答。在采用乙烯利灌心处理不同时间的过程中,发现AfFVE基因的转录水平均在处理后1 d时达到最大,推测AfFVE可能参与乙烯信号反应,该基因在粉菠萝自主开花途径中的调节作用可能受乙烯影响。FVE is a key regulator of the autonomous pathway that promote floral transition through the repression of FLC. In this study, AfFVE, a FVE homologue gene was isolated from A echmeafasciata by screening the transcriptome sequence data and RACE method. The full-length cDNA sequence of AfFVE was 1 672 bp contained a 1 404 bp open reading flame, which encodes 468 amino acids with six conserved WD repeat domains. Amino acid sequence align- ment showed that the deduced amino acid sequence of AfFVE shared high similarity with FVE homologs from other species. Quantitative real-time RT-PCR analysis showed thatAfFVE had response to ethylene in A echme afasciata. Its transcripts in shoot tips reached highest levels at 1 d after processing in different ethylene treatments. We hypothe- sized that AfFVE may be involved in ethylene signaling and its regulation in autonomous pathway may be influ- enced by ethylene.

关 键 词：粉菠萝 AfFVE 乙烯 开花 

分 类 号：S668.3[农业科学—果树学]