维生素E对HepG2脂肪变模型中caspase-3活性影响研究  

Influence of VitE on caspase-3 activity in steatosis HepG2 cells

作　　者：吴昌维[1] 陈荔萍[2] 苏大芝[1] 艾罗燕[1] 陈志威[1] 许青青[1] 江小柯[1] 王晓晗[1] 范竹萍[1] 

机构地区：[1]上海交通大学医学院附属仁济医院健康保健中心,200127 [2]长海医院,上海200433

出　　处：《国际消化病杂志》2013年第5期353-356,共4页International Journal of Digestive Diseases

基　　金：上海市公共卫生重点学科<健康教育与促进学>(12GWZX0903)

摘　　要：目的检测HepG2细胞脂肪变模型中caspase-3活性的变化,探讨抗氧化剂维生素E(VitE)对其的影响。方法 0.5 mmol/L和1 mmol/L的混合脂肪酸分别干预HepG2细胞,在不同时间点检测细胞内caspase-3活性。将1 mg/L的VitE干预脂肪变性HepG2细胞,在不同时间点检测caspase-3活性的变化。结果脂肪酸组HepG2细胞caspase-3活性较正常对照组显著升高(P<0.05),VitE干预组细胞caspase-3活性均较脂肪酸组明显下降(P<0.05)。结论脂肪酸可诱导HepG2细胞caspase-3活性升高,抗氧化剂VitE能下调脂肪变性HepG2细胞caspase-3活性从而减轻细胞损伤,这可能与氧化应激和内质网应激的改善相关。Objective To investigate the influence of fatty acid incubation on caspase-3 activity in HepG2 cells and the effect of antioxidant vitamin E on caspase-3 activity. Methods Fatty acid mixtures at a concentration of 0.5 mmol/L and 1 mmol/L were applied for the treatment of HepG2 cells respectively, and the caspase-3 activity in cells was detected at different times. Then steatosis HepG2 cells were treated by 1 mg/L vitamin E, and the caspase-3 activity was determined after intervention. Results The caspase-3 activity increased significantly after treatment of fatty acid in HepG2 ceils, while in vitamin E group, the caspase-3 activity decreased markedly （P〈 0. 05）. Conclusion The caspase-3 activity increases in fatty acid-induced steatosis HepG2 cells, and vitamin E might have protected steatosis HepG2 cells against injury by the down regulation of caspase-3 activity.

关 键 词：脂肪变性 HEPG2细胞 维生素E 氧化应激 CASPASE-3 

分 类 号：R965[医药卫生—药理学]