肿瘤细胞断层图像轮廓的三维极坐标插值方法  

3D Interpolation Method Using Contour Points in LSCM Cell Image Stack in Polar Coordinate System for 3D Reconstruction

作　　者：撒昱[1] 张莹[1] 王平[2] 庞青松[2] 李瑞健[2] 胡新华 黄彧[1] 冯远明[1,2] 

机构地区：[1]天津大学天津市生物医学检测技术与仪器重点实验室,天津300072 [2]天津医科大学肿瘤医院,天津300060 [3]East Carolina大学物理系,Greenville,NC27858,USA

出　　处：《数据采集与处理》2013年第5期620-625,共6页Journal of Data Acquisition and Processing

基　　金：国家自然科学基金(81171342,81201148)资助项目

摘　　要：利用"细胞CT"——激光扫描共聚焦显微镜可以获得肿瘤细胞三维断层图像,实现肿瘤细胞三维可视化,为此提出了一种针对细胞断层图像轮廓的三维极坐标插值方法,以图形形态学中心为极坐标原点,将轮廓点转换为由极坐标表示。按极角采样获得离散轮廓点数据,综合多层轮廓信息,依照表面轮廓变化趋势,进行均匀三次B样条拟合完成插值。针对肿瘤细胞核常见的分支核结构,利用曲率角点检测和数学形态学相结合的方法实现轮廓分割,并采用基于阈值半径的轮廓匹配方法实现分支轮廓对应,继而实现细胞分支核插值。结果显示,本方法可实现细胞及细胞核的三维插值,插值后轮廓表面三维重建表现出良好的曲面光滑性和连续性,表明极坐标插值数据可为细胞三维形态结构测量提供便利。Laser scanning confocal microscope （LSCM）,which is also called ‘Cell CT’,can be used to acquire image stacks of live cancer cells.These images are of great significance to fulfill the 3D-visualization on cell level.With regard to this study,a 3D interpolation method based on contour points is proposed in polar coordinate system.In the new method,the origin of coordinate system is chosen to be a morphological center instead of the traditional geometric center.Resample the contour points by polar angle firstly,then fit the discrete points with uniform cubic B-spline to perform the interpolation,which can integrate multi-slice counter shape information.For a common nucleus structure,branched nucleus,a special method combining image morphological methods and corner detection methods based on curvature scale space is considered to solve contour division issue,and their correspondence problem is solved through a threshold radius method.The 3D reconstruction result shows the smooth and natural surface of cells and nucleus.It indicates that the interpolation method does help for precise measurement of cell morphology and clinic study as well.

关 键 词：肿瘤细胞 分支核 极坐标 形态学方法 样条插值 

分 类 号：TP391.4[自动化与计算机技术—计算机应用技术] R730[自动化与计算机技术—计算机科学与技术]