血红蛋白在亲水石墨烯-羟基磷灰石复合膜内的直接电化学及其电催化性能研究  

Direct Electrochemistry and Electrocatalytic Activity of Hemoglobin Immobilized on Hydrophilic Graphene- Hydroxyapatite Composite Film

作　　者：侯关伟[1] 马荣娜[1] 贾丽萍[1] 姚飞[1] 霍瑞伟[1] 李娟[1] 王怀生[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《分析测试学报》2013年第10期1166-1173,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20775031;21075059);山东省泰山学者建设工程专项经费资助项目

摘　　要：将片层氧化石墨与聚苯乙烯磺酸钠反应,并于水热条件下水合肼还原快速合成亲水石墨烯片。透射电子显微镜分析显示,制得的聚苯乙烯磺酸钠改性的透明石墨烯类似波纹状的细纱。将血红蛋白(Hb)固定于羟基磷灰石和亲水石墨烯复合膜修饰电极上,研究了血红蛋白的直接电化学性质,构建了安培过氧化氢传感器。在该复合膜修饰电极上,血红蛋白表现出一对较好的氧化还原峰,其式量电位为-0.295 V,对应于Hb中FeⅢ/FeⅡ电对的氧化还原电位,且对过氧化氢的还原具有较好地催化作用,可作为传感器用于过氧化氢的检测。该生物传感器对过氧化氢的还原具有快速的安培响应(<5 s)和较低的检出限(0.58μmol/L)。Hydrophilic graphene nanosheets were rapidly synthesized by reacting graphene oxide slice layer with poly（sodium 4-styrene sulfonate）（PSS） and simultaneously reduced with hydrazine hydrate under hydrothermal conditions.High resolving transmission electron microscopy（HRTEM） analysis indicated that the PSS modified transparent graphene nanosheets are entangled with each other and resemble rippled silk.The direct electrochemistry of hemoglobin （Hb） immobilized on the HAp and hydrophilic graphene（HG） composite film was studied and an amperometric biosensor of hydrogen peroxide was developed based on the direct electron transfer of Hb.Hb entrapped in HAp-HG film displayed a pair of well-defined redox peaks at about-0.295 V（vs.SCE） in 0.1 mol/L pH 7.0 phosphate buffer solution,which corresponded to the heme FeⅢ/FeⅡ redox couples.The resulting electrode showed an excellent electrocatalytic performance to the reduction of H2O2 with a fast amperometric response of about 5 s and could be used as a H2O2 biosenor with a lower detection limit of 0.58 μmol/L.

关 键 词：血红蛋白 石墨烯 羟基磷灰石 过氧化氢 

分 类 号：O657.1[理学—分析化学] TQ123.6[理学—化学]