表面增强拉曼光谱内标法测定水杨酸浓度  

Quantitative Determination of Salicylic Acid by Surface-enhanced Raman Scattering with Internal Standard Method

作　　者：袁荣辉[1] 滕渊洁[1] 刘文涵[1] 马淳安[1] 

机构地区：[1]浙江工业大学化学工程与材料学院,绿色化学合成技术国家重点实验室培育基地,浙江杭州310032

出　　处：《分析测试学报》2013年第10期1247-1250,共4页Journal of Instrumental Analysis

基　　金：浙江省公益性应用研究计划项目/分析测试基金(2012C37014);浙江省重点科技创新团队资助项目(2011R09002-12)

摘　　要：采用电化学方法处理银电极表面,使之产生具有表面增强拉曼光谱（SERS）的活化效应,进而利用水杨酸（SA）吸附于粗糙活化的银电极表面所产生的SERS光谱,进行SA含量的测定.选择硫氰化钾（KSCN）为内标物,以SA的苯环呼吸振动峰（1 036 cm-1）与SCN-的特征峰（2 120 cm-1）强度比值作为相对强度,其相对强度与水杨酸的浓度在4×10-6～3×10-4 mol/L范围内呈良好的线性关系,其线性系数为0.993 1;检出限为2.04×10-6 mol/L;加标回收率为85.4％～114.6％.方法具有测定简便、内标物干扰小、绿色无害等特点,可进一步推广于其他特殊物质的测定.Surface-enhanced Raman scattering （SERS） presents a significant enhanced effect.The surface of Ag electrode was pretreated by electrochemical method to obtain a SERS active substrate.The concentration of salicylic acid （SA） absorbed on this rough Ag electrode could be determined by the corresponding SERS spectra.Potassium thiocyanate was selected as the internal standard and the relative intensity was formed by aromatic ring breathing（1 036 cm-1） of SA and characteristic peak（2 120cm-1） of SCN-.A good linear relationship was obtained between the relative intensity and concentration of SA in the range of 4 × 10-6--3 × 10-4 mol/L,with a correlation coefficient of 0.993 1.The detection limit was 2.04 × 10-6 mol/L and the recoveries were in the range of 85.4％-114.6％.The interference experiments were also considered.With the advantages of convenience,no interference of internal substance,green and harmlessness,the method could be extended to the determination of other special substances.

关 键 词：表面增强拉曼光谱 内标法 水杨酸 银电极 

分 类 号：O657.37[理学—分析化学]