HPLC-UV-MS法对洋蓟中多酚类化合物的分析和鉴定  

Analysis and Identification of Polyphenols in Artichoke(Cynara scolymus L.) by the Method of HPLC-UV-MS

作　　者：郑成凤[1] 潘裕添[2] 苑小宁[1] 高飞[1] 

机构地区：[1]闽南师范大学化学与环境科学系,福建漳州363000 [2]闽南师范大学菌物产业工程技术中心,福建漳州363000

出　　处：《漳州师范学院学报（自然科学版）》2013年第3期81-87,共7页Journal of ZhangZhou Teachers College（Natural Science)

基　　金：福建省高校产学合作科技重大项目基金(2012N5013);漳州师范学院科研启动基金(L20476)

摘　　要：借助HPLC-UV-MS方法对云南洋蓟中多酚类化合物进行分析鉴定和含量测定.液相色谱分离采用ZORBAX Eclipse XDB-C18分析柱,以乙腈和水(含0.08%甲酸)为流动相梯度洗脱,流速为0.5 mL/min.根据多级质谱分析,并与对照品和文献数据比较,确定洋蓟样品甲醇提取液中含有19种多酚类化合物.色谱实验结果表明绿原酸、木犀草苷和水飞蓟宾在其线性范围内具有良好的线性关系(R2≥0.9998),最低检测限(LOD)分别为0.153μg/mL、0.336μg/mL和0.313μg/mL,且在洋蓟样品中的含量分别为27.79 mg/g、10.25 mg/g和58.72 mg/g.In this paper, we conducted an analysis identification and content determination of polyphenolic compounds in artichoke （Cynara scolymus L.） from Yunnan by the method of HPLC-UV-MS. The liquid chromatographic separation was performed by the ZORBAX Eclipse XDB-C18 analytical column, which based in the mobile phase gradient elution with acetonitrile and water （containing 0.08%formic acid） at a flow rate of 0.5 mL/min. According to the analysis of multistage mass spectrometry, and the comparison with reference compounds and literature data, we confirmed that methanol extract in artichoke samples contained 19 kinds of polyphenolic compounds. Chromatographic experimental results showed that chlorogenic acid, luteoloside and silibinin had a good linear regression （R2≥0.9998） in its linear ranges, and the limits of detection （LODs） were 0.153μg/mL、0.336μg/mL and 0.313μg/mL, respectively. And the content in artichokes sample were 27.79 mg/g, 10.25 mg/g and 58.72 mg/g, respectively.

关 键 词：洋蓟 多级质谱 高效液相色谱 多酚类化合物 

分 类 号：R284.1[医药卫生—中药学]