洛伐他汀对TNF-α诱导人脐静脉内皮细胞MCP-1和IL-10表达的影响  

Influence of lovastatin in expressions of MCP-1 and IL-10 of HUVECs treated with TNF-α

作　　者：赵铁柱[1] 沈焕[1] 陶致侃[1] 王为[1] 赵丹丹[1] 

机构地区：[1]沈阳医学院沈洲医院血管外科,辽宁沈阳110032

出　　处：《吉林大学学报（医学版）》2013年第5期928-932,共5页Journal of Jilin University：Medicine Edition

基　　金：辽宁省教育厅科学技术研究项目资助课题(2008848)

摘　　要：目的:观察洛伐他汀(LVT)对肿瘤坏死因子α(TNF-α)刺激人脐静脉内皮细胞(HUVECs)后单核细胞趋化蛋白-1(MCP-1)和白细胞介素-10(IL-10)表达的影响,探讨其抗动脉粥样硬化(AS)的可能机制。方法:体外常规培养HUVECs,分为对照组,用含10%FBS的RPMI 1640培养液培养;TNF-α处理组,用含10%FBS的RPMI 1640培养液+TNF-α(20μg·L-1)处理;应用活性氧检测试剂盒检测活性氧含量;LVT干预组,用含10%FBS的RPMI 1640培养液+TNF-α(20μg·L-1)+LVT(10μmol·L-1)处理。实时定量RT-PCR方法检测各组细胞MCP-1和IL-10mRNA的表达;Western blotting方法检测各组HUVECs内MCP-1、IL-10和NF-κB蛋白表达。结果:与对照组相比较,TNF-α处理组ROS含量明显增高(P<0.05);与TNF-α处理组比较,LVT干预组ROS含量明显降低(P<0..05);与对照组比较,TNF-α处理组MCP-1和NF-κB mRNA及蛋白表达水平明显增高(P<0.05),而IL-10mRNA和蛋白的表达水平明显降低(P<0.05);与TNF-α处理组比较,LVT干预组细胞MCP-1和NF-κB mRNA及蛋白表达水平明显降低(P<0.05),而IL-10mRNA和蛋白表达水平明显增高(P<0.05)。结论:LVT抑制HUVECs中TNF-α诱导的MCP-1mRNA和蛋白上调,增强IL-10蛋白表达。LVT可能通过NF-κB途径对抗炎症反应从而防止AS的形成。Objective To investigate the potential anti-atherosclerosis（AS）mechanism of lovastatin by observing the influence of lovastatin on the expressions of monocyte chemotactic protein-1（MCP-1）and interleukin 10（IL-10） in human umbilical vein endothelial cells（HUVECs）treated with tumor necrosis factor-α（TNF-α）.Methods The HUVECs were routinely cultured in the medium RPMI 1640containing 10%fetal bovine serum（FBS）in vitro and divided into 3groups：control group,TNF-αtreated group（RPMI 1640medium ＋TNF-α（20μg·L-1）,and lovastatin treated group（RPMI 1640medium ＋20μg·L-1 TNF-α＋10μmol·L-1 Lovastatin）;Application kit to detect ROS content;Real-time RT-PCR was used to measure the mRNA expressions of MCP-1and IL-10in HUVECs in each group;Western blotting method was used to analyze the protein expressions of MCP-1,NFκB and IL-10in HUVECs in each group.Results Compared with the control group,the expressions of ROS in TNF-α treated group were enhanced obviously（P0.05）.Compared with TNF-αtreated group,the ROS expressions in the lovastatin treated group were decreased significantly（P0.05）.Compared with control group,the mRNA and protein expression levels of MCP-1and NFκB in TNF-αtreated group were enhanced obviously（P0.05）,and the mRNA and protein expression levels of IL-10 were decreased statistically（P0.05）.Compared with TNF-α treated group,the mRNA and protein expression levels of MCP-1and NFκB in lovastatin treated group were decreased significantly（P0.05）,but the expression level of IL-10 was increased statistically（P 0.05）.Conclusion Lovastatin can inhibit the up-regulation of MCP-1and NFκB and increase the expression of IL-10in the HUVECs treated with TNF-α.Lavastatin may inhibit inflammation and prevent artherosclerosis through NF-κB pathway.

关 键 词：人脐静脉内皮细胞 单核细胞趋化蛋白1 白细胞介素10 动脉粥样硬化 洛伐他汀 

分 类 号：R543[医药卫生—心血管疾病]