长春地区部分医院革兰阴性杆菌16S rRNA甲基化酶基因的检测及其意义  

Detection of 16S rRNA methylase genes in gram-negative bacilli isolated from hospitals in Changchun area and its significance

作　　者：周佳琦[1] 史红艳[1] 徐花[1] 黄金凤 孙延波[1] 

机构地区：[1]吉林大学白求恩医学院病原生物学系,吉林长春130021 [2]辽宁省大连市科兴疫苗技术有限公司,辽宁大连116620

出　　处：《吉林大学学报（医学版）》2013年第5期933-937,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省发改委省校合作技术开发项目资助课题(3R2097783426)

摘　　要：目的:分析长春地区部分医院革兰阴性杆菌16S rRNA甲基化酶基因的分布和类型,为氨基糖苷类抗生素的合理应用提供依据。方法:采用琼脂稀释法筛选出阿米卡星与庆大霉素的耐药株,通过聚合酶链式反应(PCR)扩增耐药菌株所携带的16S rRNA甲基化酶基因及其类型(armA、rmtB、rmtA、rmtC、rmtD和npmA),利用基因测序比对扩增基因序列并确定16S rRNA甲基化酶基因类型。结果:116株革兰阴性杆菌对阿米卡星和庆大霉素的耐药率分别为42.2%和75.0%,其中50株革兰阴性杆菌呈16S rRNA甲基化酶基因阳性(43.1%,50/116),其主要类型为armA和rmtB(检出率分别为12.1%和31.0%),而rmtA、rmtC、rmtD和npmA的检出率均为0。结论:长春地区革兰阴性杆菌16SrRNA甲基化酶基因主要以armA和rmtB 2种类型存在,是其导致氨基糖苷类抗生素高水平耐药的原因之一。Objective To analyze the distribution and genotypes of 16SrRNA methylase genes in gram-negative bacilli isolated from hospitals in Changchun area and to provide a reasonable basis for the application of aminoglycosides.Methods Amikacin-resistant and gentamicin-resistant strains were screened using agar dilution tests,and 16SrRNA methylase genes in gram-negative bacilli isolates were amplified by PCR and genotypes of 16SrRNA methylases（armA,rmtB,rmtA,rmtC,rmtD and npmA）were analyzed using gene sequencing.Results The resistant rates of 116clinical isolates of gram-negative bacilli to amikacin and gentamicin were 42.2% and 75.0%,respectively.Fifty of 116isolates were positive for 16SrRNA methylase genes（43.1%,50/116）.The common genotypes of 16SrRNA methylase were armA and rmtB（12.1%and 31.0%）;however,the rmtA,rmtC,rmtD and npmA genes were not found.Conclusion The genotypes of 16SrRNA methylase in gram-negative bacilli in Changchun area are armA and rmtB which is one of the reasons to induce high resistance to aminoglycosides.

关 键 词：革兰阴性杆菌 16S rRNA甲基化酶 耐药性 

分 类 号：R378[医药卫生—病原生物学]