盐溶法构建磨损颗粒诱导小鼠颅骨溶解模型方法的建立及评价  

Establishment and evaluation of mouse skull dissolvement model induced by wear particles with salt solution method

作　　者：刘铭[1] 魏小兰[1] 陈婷梅[2] 姜蓉[3] 张健[4] 

机构地区：[1]安康职业技术学院附属医院,陕西安康725000 [2]重庆医科大学检验系临床检验诊断学教育部重点实验室,重庆400016 [3]重庆医科大学干细胞与组织工程研究室免疫组织化学室,重庆400016 [4]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《吉林大学学报（医学版）》2013年第5期1041-1044,I0004,共5页Journal of Jilin University：Medicine Edition

基　　金：重庆市科委研究专题资助课题(CSTC;2010AB5094);重庆市科技攻关计划项目资助课题(CSTC2010AB5094)

摘　　要：目的:探讨构建新型磨损颗粒诱导的骨溶解模型,为研究人工关节无菌性松动提供简单、方便的实验动物模型。方法:制备无菌盐包埋磨损颗粒;采用随机数字表法将50只BALB/c小鼠分为空白对照组、纯盐组、气囊植骨组和盐包埋磨损颗粒组,其中气囊植骨组20只,其他各组10只;气囊植骨组小鼠术后21d,余3组术后14d获取小鼠颅骨组织标本,HE染色观察炎细胞渗出量和骨溶解面积;扫描电镜观测骨片吸收陷窝面积百分比;抗酒石酸酸性磷酸酶(TRAP)染色检测破骨细胞增殖分化。结果:HE染色,与空白对照组和纯盐组比较,气囊植骨组和盐包埋磨损颗粒组小鼠的炎性细胞渗出量、骨溶解面积明显增大(P<0.01);与气囊植骨组比较,盐包埋磨损颗粒组小鼠TRAP染色破骨细胞数量明显增多(P<0.01);扫描电镜观察,盐包埋磨损颗粒组小鼠骨片吸收陷窝面积百分比显著高于气囊植骨组(P<0.01)。结论:盐溶法是一种简单、经济、快速准确的构建小鼠颅骨溶解模型方法,能够真实模拟人工关节松动发生的病理过程。Objective To build a new type of wear particle induced bone dissolution model,and to provide a simple and convenient aseptic loosening experimental animal model for the study of artificial joints.Methods A total of 50BALB/c mice were randomly divided into blank control group,pure salt group（n=10）,airbag bone graft group（n=10）,and salt embedding wear particle group（n=10）.The skull bone samples of mice in airbag bone graft group and other groups were obtained 21and 14dafter operation,respectively.The number of inflammatory cells and osteolysis area were observed by HE staining.The percentage of bone absorption lacuna area was detected by scanning electron microscope.The proliferation and differentiation of osteoclasts were observed by TRAP staining Results The HE staining results showed that compared with airbag bone graft group and black group,the efflux of inflammatory cells and osteolysis areas in pure salt group and wear particle group were increased significantly（P 0.01）.The TRAP staining results showed that the number of osteoclasts in airbag bone graft group was significantly lower than that in wear particle group（P0.01）.The scanning electron microscope results showed that the percentage of bone absorption lacuna area in wear particle group was significantly higher than that in airbag bone graft group（P0.01）.Conclusion Salt solution method is a simple,economic,and fast method to build myuse skull dissolvement models,and it can accurately simulate the pathological process of aseptic loosening after artificial joint replacement.

关 键 词：磨损颗粒 颅骨溶解 无菌松动 气囊植骨 小鼠 近交BALBc 

分 类 号：R332[医药卫生—人体生理学]