失血复合氰化物中毒对大鼠脑血氧和能量代谢的影响  

Effects of cyanide compounds on cerebral oxygen and energy metabolism in rats' brain under hemorrhagic shock

作　　者：刘辉[1] 张宇辉[1] 尹芳秋[1] 贾庆军[1] 高秋菊[1] 边巍[1] 

机构地区：[1]中国人民解放军白求恩医务士官学校卫勤教研室,石家庄050081

出　　处：《现代预防医学》2013年第21期4035-4037,4039,共4页Modern Preventive Medicine

基　　金：全军医学科技"十二五"科研基金面上项目(CWS11J156)

摘　　要：目的观察失血复合氰化物中毒对大鼠脑血氧和能量代谢的影响。方法实验动物随机分为假手术组、失血性休克组、氰化钾中毒组和失血性休克复合氰化钾中毒组,观察各组大鼠脑静脉血氧饱和度(jugular venous O2saturation,SjvO2)的改变,采用高效液相色谱测定大脑皮层ATP、ADP和AMP含量,分光光度法检测大脑皮层ATP酶活性的变化。结果与氰化钾中毒组比较,休克复合氰化钾中毒组大鼠SjvO2下降程度更为严重,且恢复速度减慢;脑ATP含量在复合损伤60 min和120 min时降低,差异有统计学意义,ADP、AMP含量则明显升高;脑Na+-K+-ATPase和Ca2+-ATPase活性变化趋势与单纯中毒组一致,但在复合损伤60 min和120 min时活性低于氰化钾中毒组,差异有统计学意义。结论失血条件下氰类化合物中毒可进一步加重脑血氧和能量代谢障碍。OBJECTIVE To investigate effects of cyanide compounds on cerebral oxygen and energy metabolism in rats＇ brain under hemorrhagic shock. METHODS SD rats were randomizedly divided equally into sham operation group （SO）, hemorrhagic shock group （HS）, potassium cyanide intoxication group （KCN） and hemorrhagic shock combined potassium cyanide intoxication group （HS＋KCN）. Blood samples were taken from internal jugular bulb to determine SjvO2. The contents of ATP, ADP and AMP in rats＇ cortex were assayed by high performance liquid chromatography （HPLC）. The activities of Na＋-K＋-ATPase and Ca2＋-ATPase were measured by usingwith ATPase detect kit. RESULTS Compared with KCN group, SjvO2 obviously decreased in （HS＋KCN） group, ATP contents significantly decreased, but ADP and AMP content increased. The activities of Na＋-K＋-ATPase and Ca2＋-ATPase in rats＇ cortex were obviously inhibited. CONCLUSION Hemorrhagic shock could markedly disturb cerebral oxygen and energy metabolism in rats＇ brain intoxicated by KCN.

关 键 词：失血性休克 氰化钾 血氧饱和度 ATP ATP酶 

分 类 号：R595[医药卫生—内科学] R743.9[医药卫生—临床医学]