基于中红外光谱的牛蒡子饮片中牛蒡苷和牛蒡苷元快速含量测定方法的建立  

Rapid determination method of the contents of arctiin and arctigenin in Fructus Arctii by mid-infrared spectrometry

作　　者：杜伟锋[1] 鲍雯雯[1] 赵耀东[1] 郭昊[1] 王胜波[1] 丛晓东[1] 

机构地区：[1]浙江中医药大学中药炮制技术研究中心,杭州311401

出　　处：《中国新药杂志》2013年第20期2431-2434,2460,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金青年科学基金项目(81303224);浙江省中医药科学研究基金计划(20122B032;2013ZA027);浙江省教育厅科研计划(Y20122449)

摘　　要：目的:应用中红外光谱分析技术,建立快速检测牛蒡子中牛蒡苷和牛蒡苷元含量的新方法。方法:用HPLC法测定牛蒡苷和牛蒡苷元的含量,采用偏最小二乘法(PLS),建立中红外光谱与HPLC法测定值之间的多元校正模型,并预测牛蒡子生品和炮制品中牛蒡苷和牛蒡苷元的含量。结果:牛蒡子生品中牛蒡苷和牛蒡苷元的校正模型的相关系数(r)、校正标准偏差(RMSEC)、预测标准偏差(RMSEP)分别为0.998 9,0.068 7,0.951 0和0.999 5,0.022 0,0.322 3;牛蒡子炒制品中牛蒡苷和牛蒡苷元的校正模型的相关系数、校正标准偏差、预测标准偏差分别是0.982 4,0.206 1,1.010和0.995 8,0.069 4,0.406 8。经外部验证,预测值与真实值的相对偏差较小。结论:该方法准确、快速、简便,可实现大批量样品的快速分析。Objective：To develop a mid-infrared quantitative method for rapid determination of arctiin and arctigenin in Fructus Arctii. Methods：Mid-infrared diffuse reflectance spectroscopy technology was used to collect mid-infrared spectra of Fructus Arctii with HPLC analysis values as reference, and the rapid determination method of arctiin and arctigenin was established with partial least squares （PLS）. Results：The correlation coefficients （r）, root-mean-square error of calibration （RMSEC）and root-mean-square error of validation （RMSEP） of the calibra- tion model for arctiin and arctigenin in Fructus Arctii crude drug were 0. 998 9,0. 068 7,0. 951 0 and 0. 999 5, 0. 022 0,0. 322 3 ,respectively;these parameters of arctiin and arctigenin in Fructus Arctii processed were 0. 982 4, 0. 206 1,1. 010 and 0. 995 8,0. 069 4,0. 406 8 ,respectively. The relative standard deviations（RSDs） of the true value and prediction value from external validation of crude and processed Fructus Arctii were reasonable. Conclusion ：The method is accurate,rapid, and simple, and could be applied for the rapid determination of a large number of samples.

关 键 词：傅里叶变换中红外光谱 牛蒡子 牛蒡苷 牛蒡苷元 

分 类 号：R927.2[医药卫生—药学]