通络止痛合剂对GA大鼠MMP-3、IFN-γ的影响  

Influence of Tongluo Zhitong Mixture on MMP-3 and IFN-(?) in Gouty Arthritis Model Rats

作　　者：李东书[1] 姚岚[1] 孟庆一[1] 

机构地区：[1]沈阳市中医院风湿科,辽宁沈阳110004

出　　处：《中华中医药学刊》2013年第11期2517-2519,I0009,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：沈阳市科技局自然科学基金资助项目(F11-262-953)

摘　　要：目的:观察通络止痛合剂对痛风性关节炎大鼠基质金属蛋白酶-3(MMP-3)、γ-干扰素(IFN-γ)的影响。方法:应用Coderre经典造模方法造模,予通络止痛合剂治疗,并以秋水仙碱、醋氯芬酸为对照,观察大鼠的一般状态、受试关节步态、关节肿胀周径及组织病理学的变化;采用ELISA法,检测各组大鼠受试关节内MMP-3、IFN-γ的含量;同时观察各治疗组对小鼠扭体实验的镇痛作用。结果:各治疗组均能明显改善关节滑膜炎症的病理改变、降低关节内MMP-3及IFN-γ的含量和减轻醋酸引起的小鼠疼痛(P<0.05);但各治疗组之间相比无差异(P>0.05)。结论:通络止痛合剂具有良好的消肿止痛作用、并通过抑制关节内MMP-3及IFN-γ的活性,减轻关节组织的炎症反应,以治疗痛风性关节炎。Objective ： To observe the effect of Tongluo Zhitong Mixture on matrix metalloproteinase - 3 （ MMP - 3）, interferon gamma （ IFN - -γ） of gouty arthritis rats. Methods ： Coderre classic modeling methods was used to establish the model which was treated with Tongluo Zhitong Mixture, and with colchicine, aceclofenac as control, rats were observed for the general state, the variations of joint gait, swelling of joint circumference and histopathology changes. The method of ELISA was used to test the contents of MMP - 3 and IFN - γ of rats ; at the same time all treatment groups analgesia on mice was observed by writhing test. Results ： Pain in mice in each treatment group can improve the pathological changes in synovitis,reduce the contents of MMP- 3 and IFN-γ in joint and reduce pain induced by acetic acid （P 〈 0.05 ） ;but compared with the model group, the treatment group had no difference （P 〉 0. 05）. Conclusion ： Tongluo Zhitong Mixture has good swelling analgesic effect, and through inhibition of MMP - 3 and IFN - γ activity, it can reduce inflammation of the joint tissue in the treatment of gouty arthritis.

关 键 词：通络止痛合剂 痛风性关节炎 基质金属蛋白酶-3 Γ-干扰素 

分 类 号：R332[医药卫生—人体生理学]