Toll 样受体9与系统性红斑狼疮患者的临床特点相关性  

Correlation between the Toll-like Receptor 9 and Clinical Features of Systemic Lupus Erythematosus Patients

作　　者：史晓飞[1] 王可[1] 樊琳琳[1] 马新[1] 付建斌[1] 

机构地区：[1]河南科技大学第一附属医院风湿免疫科,洛阳471003

出　　处：《中华临床免疫和变态反应杂志》2013年第3期224-227,共4页Chinese Journal of Allergy & Clinical Immunology

基　　金：洛阳市科技发展计划(1002237B)

摘　　要：目的探讨Toll样受体9(toll-like receptor 9,TLR9)在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者中的表达情况及其与临床的关系。方法采用细胞内流式细胞术对48例SLE组、36例疾病对照组(包括类风湿关节炎、干燥综合征、系统性硬化症、肌炎皮肌炎)和25名健康对照组患者外周血B淋巴细胞中TLR9表达情况进行比较,并记录SLE患者临床表现和实验室检查,对TLR9的表达与临床特点的相关性进行分析。结果 SLE活动期组外周血CD19+B细胞TLR9表达率(54.18±17.97)高于SLE非活动期(32.97±9.85)、疾病对照组(30.88±9.12)及健康对照组(29.75±7.49)(均P<0.01)。且其表达率与SLE疾病活动指数评分(r=0.763)、抗双链DNA抗体滴度(r=0.781)呈正相关(均P<0.01)。应用中小剂量糖皮质激素对SLE活动期患者TLR9在CD19+B细胞的表达无显著差异(P>0.05)。结论 TLR9参与了SLE的发病,可以作为判断疾病活动性及临床预后的指标。Objective To investigate the expression of Toll-like receptor 9 （TLR9） in patients with systemic lupus erythematosus（SLE）, and analyze its association with disease activity. Methods The expressions of TLR9 on peripheral blood CD19＋ B lymphocyte were measured by flow cytometry respectively in 48 SLE patients, 36 patients with other connective tissue disease and 25 healthy individuals. Moreover, the correlations between expressions of TLR9 and the laboratory and clinical features were analyzed. Results Intracellular TLR9 expression levels of B lymphocytes in SLE patients with active disease （54. 18±17.97 ） were significantly higher than in SLE patients with inactive disease （32. 97 ± 9. 85 ）, the disease controls （30. 88± 9. 12） and the healthy controls （29. 75 ±7. 49） （ all P 〈0. 01 ）. The positive correlation was also observed between TLR9 expression levels and the SLEDAI （r =0. 763）, and anti-dsDNA （r =0. 781 ） （all P 〈0. 01 ）. In addition, the increased expression of TLR9 in B cells did not change significantly after treatment with corticosteroids （P 〉 0. 05）. Conclusion TLR9 may help in assessing disease activity and prognosis in SLE patients.

关 键 词：TOLL样受体9 系统性红斑狼疮 相关性 

分 类 号：R593.241[医药卫生—内科学]