Enhancement of Laccase Activity by Marine-derived Deuteromycete Pestalotiopsis sp. J63 with Agricultural Residues and Inducers  

作　　者：冯晓雨 陈慧英 薛栋升 姚善泾 

机构地区：[1]Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biologi-cal Engineering, Zhejiang University, Hangzhou 310027, China

出　　处：《Chinese Journal of Chemical Engineering》2013年第10期1182-1189,共8页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （21036005） and Scientific and Technology Plan of Zhejiang Province （2011C33016）.

摘　　要：Pestalotiopsis sp. J63, producing a high activity of laccase, is a new marine-derived fungus isolated from the oceanic sediment of the East China Sea. Since the marine environment is oligotrophic nutrient, marine derived fungi may use small amount of nutrients to grow and produce laccases. Agricultural residues that are mainly composed of lignin, cellulose and hemicellulose are difficult to be degraded and few microbes can take them as substrates, so they are considered as oligotrophic nutrient and have the potential to be used to produce value added products. In this study, the ability of Pestalotiopsis sp. J63 to use agricultural residues to produce laccases was tested in the submerged fermentation. The combination of 3 g·L 1maltose and 20 g·L 1rice straw was the best carbon sources and 8 g·L 1ammonium sulfate was the best nitrogen source under the condition without inducers. The effects of five inducers, the feeding time and concentration of inducer on laccase production were investigated.Adding 0.09 mmol·L 1phenol after 24 h of incubation led to high laccase activity(5089 U·L 1), while with 0.09mmol·L 1phenol in the medium and wheat bran as the nitrogen source, the laccase activity could reach 5791.7U·L 1. Native-PAGE results showed that two laccase isozymes were present in the cultures. One existed in both induced and non-induced culture filtrates, while the other was only found in the fermentation with the addition of phenol, guaiacol and veratryl alcohol.Pestalotiopsis sp. J63, producing a high activity of laccase, is a new marine-derived fungus isolated from the oceanic sediment of the East China Sea. Since the marine environment is oligotrophic nutrient, marine de-rived fungi may use small amount of nutrients to grow and produce laccases. Agricultural residues that are mainly composed of lignin, cellulose and hemicellulose are difficult to be degraded and few microbes can take them as sub-strates, so they are considered as oligotrophic nutrient and have the potential to be used to produce value added products. In this study, the ability of Pestalotiopsis sp. J63 to use agricultural residues to produce laccases was tested in the submerged fermentation. The combination of 3 g·L-1 maltose and 20 g·L-1 rice straw was the best car-bon sources and 8 g·L-1 ammonium sulfate was the best nitrogen source under the condition without inducers. The effects of five inducers, the feeding time and concentration of inducer on laccase production were investigated. Adding 0.09 mmol·L-1 phenol after 24 h of incubation led to high laccase activity （5089 U·L-1）, while with 0.09 mmol·L-1 phenol in the medium and wheat bran as the nitrogen source, the laccase activity could reach 5791.7 U·L-1. Native-PAGE results showed that two laccase isozymes were present in the cultures. One existed in both in-duced and non-induced culture filtrates, while the other was only found in the fermentation with the addition of phenol, guaiacol and veratryl alcohol.

关 键 词：laccase isozyme Pestalotiopsis sp. marine microorganism carbon and nitrogen source inducer 

分 类 号：X71[环境科学与工程—环境工程] P736.21[天文地球—海洋地质]