高羊茅单株耐热性相关分子标记的筛选及其与越夏性的关系研究  

Screening molecular markers for heat tolerance and its relation to summer tolerance in tall fescue single plants

作　　者：陈群[1] 袁晓君[2] 何亚丽[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海大学生命科学技术学院,上海200444

出　　处：《草业学报》2013年第5期84-95,共12页Acta Prataculturae Sinica

基　　金：国家自然科学基金面上项目(30972134)资助

摘　　要：筛选与高羊茅单株耐热性和越夏性相关的分子标记有利于建立高效育种体系。以来自于耐热性不同的12个高羊茅种质资源的216个单株为耐热性分子标记筛选的基础群体,在人工高温(40℃/35℃,昼/夜各12h)和夏季田间自然条件下分别进行了耐热性和越夏性鉴定。运用简单重复序列区间(inter-simple sequence repeat,ISSR)和随机扩增多态性DNA(random amplified polymorphism DNA,RAPD)分子标记技术,采用改良的集团分离分析法(modified bulked segregant analysis,BSA),从800个RAPD和100个ISSR分子标记中筛选与耐热性相关分子标记,并研究其与单株越夏性的关系。结果表明,单株的耐热性与越夏性的相关程度较低(决定系数为3.4%);引物351扩增的750bp条带(351-T750)与耐热性显著相关,而引物138扩增的950bp条带(138-T950)则与耐热性和越夏性均显著相关。可以推论,耐热性强和/或越夏性强单株材料需要分别在人工高温和夏季田间自然条件下加以鉴定筛选;351-T750可应用于耐热性强单株的辅助选择,而138-T950则可以用于耐热性强与越夏性强单株的辅助选择。To establish an efficient breeding system in tall fescue （Festuca arundinacea）, molecular markers re- lated to heat and summer tolerances were screened for marker-assisted selection breeding. Initially, 216 single plants from 12 germplasms differing in heat tolerance were used as a basic population to identify their heat stress （40~C/35 C, day/night, 12 h/12 h） tolerance in an artificial climate chamber and their summer stress tolerance in experimental fields in Shanghai. Random amplified polymorphism DNA （RAPD） and inter-simple sequence repeat （ISSR） were assessed with modified bulked-segregant analysis （BSA） to screen for molecular markers among the 800 RAPD and 100 ISSR primers. The correlation between heat and summer tolerance among single plants was quite low with only 3.4o/oo of the determination coefficient; Primer 351 amplified a 750 bp band （351-T750） which was significantly related to heat tolerance, while 138 amplified a 950 bp band related to both heat and summer tolerance. Heat and summer tolerant single plants should be screened under artificial heat and natural summer stresses respectively; Band 351-T750 could be used only for heat tolerant plant selection, while 138-T950 could be used for both heat and/or summer tolerant plant selection.

关 键 词：高羊茅 耐热性 越夏性 简单重复序列区间分子标记(ISSR) 随机扩增多态性DNA(RAPD) 集团分离分析法(BSA) 

分 类 号：S688.4[农业科学—观赏园艺]