9种暖季型草坪草耐阴性综合评价及其指标的筛选  

Evaluation of shade tolerance of nine warm-season turfgrass and selection of their shade tolerant indices

作　　者：罗耀[1,2] 席嘉宾[3] 谭筱弘 张巨明[2] 

机构地区：[1]广州市园林科学研究所,广东广州510405 [2]华南农业大学南方草业中心,广东广州510642 [3]中山大学生命科学院,广东广州510275 [4]广西来宾市兴宾区水产畜牧兽医局,广西来宾546100

出　　处：《草业学报》2013年第5期239-247,共9页Acta Prataculturae Sinica

基　　金：广东省科技攻关项目(2012B020302002);广州市财局项目(2061200000413)资助

摘　　要：对9种暖季型草坪草进行遮阴逆境胁迫研究,测定了叶面积、叶片长度、叶片长宽比、节间距离、地上生物量、叶绿素a含量、叶绿素b含量、叶绿素总量、可溶性糖含量、POD活性、Fv/Fm、Fv/Fo等12个形态和生理生化指标,并把各个指标数据转化为遮阴条件下与对照(全光照)的相对值,采用相关分析和主成分分析,将12个指标转化为4个相互独立的综合变量,求得各品种的每一个综合指标值及相应的隶属函数值,经过综合加权进行综合评价,得出9种草坪草的耐阴性强弱排序是:中大1号地毯草、钝叶草、金边钝叶草、兰引Ⅲ号结缕草、海滨雀稗、普通地毯草、近缘地毯草、假俭草、沟叶结缕草。利用逐步回归建立最优回归方程,筛选出叶片长宽比、节间距离、叶绿素a含量、叶绿素b含量和过氧化物酶活性等对草坪草耐阴性有显著影响的5个指标,可作为评价草坪草耐阴性的指标,使评价工作简单化。本研究采用生理形态指标的数理分析对草坪草耐阴性进行综合评价,该方法较为客观全面,可以为草坪草的抗逆性研究提供参考和借鉴。Shade tolerance of nine warm-season turfgrass was carried out by evaluating 12 morphological and physiological indices： leaf area, leaf length, leaf length to width ratio, internode length, biomass, chlorophyll a content, chlorophyll b content, total chlorophyll content, soluble sugar, POD activity, Fv/Fm and Fv/Fo, all of which are closely correlated with shade tolerance. Each index value was converted into the relative value of shade tolerance compared with the full light control. Using correlation analysis and principal component a- nalysis, the 12 indices were transformed into four mutually independent comprehensive variables, and then each index value and its corresponding subordinate function value were calculated. After a comprehensive eval- uation of each varieties shade tolerance, the rank of the nine turfgrass shade-tolerance from strong to weak was： Axonopus compressus cv. Zhongda 1, Stenotaphrum helferi , Stenotaphrurn helferi ＇golden＇, Zoysia ja- ponica cv. Lanyin Ill , Paspalurn vaginatum, Axonopus cornpressus, Axonopus affinis, Erernochloa ophiu- roides and Zoysia rnatrella. Using stepwise regression analysis, the regression equation was established and five indices （leaf length to width ratio, internode length, chlorophyll a content, chlorophyll b content and POD activity） were selected to evaluate shade tolerance of turfgrass as tolerance significantly influenced these indices thereby simplifying the evaluation process. It was concluded that the mathematical analysis method of using morphological and physiological indices data in the comprehensive evaluation of turfgrass shade tolerance was relatively objective and comprehensive, and can be used in turfgrass resistance studies.

关 键 词：草坪草 耐阴性 综合评价 评价指标 

分 类 号：S688.4[农业科学—观赏园艺]