恒河猴、比格犬、树鼩、兔、大鼠及小鼠消化管的比较组织学  

Comparison histologic study on digestive tract of rhesus monkey,beagle dog,japanese rabbit,tree shrew,SD rat and KM mouse

作　　者：徐文漭[1] 李霞[2] 李涛[1] 王媛媛[1] 戴芳[1] 和占龙[3] 王丽[1] 蔡琳[1] 黎贵芸[1] 崔静[1] 杨举伦[1] 

机构地区：[1]成都军区昆明总医院病理科,昆明650032 [2]成都军区昆明总医院全军创伤骨科研究所,昆明650032 [3]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《临床与实验病理学杂志》2013年第11期1211-1217,共7页Chinese Journal of Clinical and Experimental Pathology

基　　金：云南省科技基础条件平台建设项目(2005PT05);云南省应用基础研究自筹项目(2011FZ323)

摘　　要：目的对恒河猴、比格犬、树鼩、日本大耳白兔、SD大鼠及昆明小鼠(KM小鼠)的消化管进行比较组织学研究,为实验动物病理学检测标准及以它们为基础的科学研究提供理论依据。方法选取按照现行实验动物质量国家检测标准检测合格的恒河猴30只、比格犬16只、树鼩20只、日本大耳白兔18只、SD大鼠20只及KM小鼠20只,经麻醉后处死并剖检。选取消化管组织经10%中性福尔马林固定,常规石蜡切片,行HE、免疫组化及特殊染色,光镜观察,比较它们在组织学结构方面的异同。结果所有动物直肠的组织学结构基本一致,下列部位组织学存在差异:(1)舌肌的排列方式及舌腺的组成类型,舌乳头发达程度及其内味蕾的多少;(2)食管黏膜表面上皮形态、有无食管腺及其组成类型、肌层的排列方式;(3)胃的大体结构,有无贲门腺、胃底腺及幽门腺等组织学结构,胃底表面上皮细胞形态及AB染色情况;(4)十二指肠、空肠及回肠黏膜下层的组织学结构;(5)结肠黏膜层组织学结构及部分实验动物的特有结构;(6)炎症是消化管普遍存在的自发性病变。结论 (1)通过对上述实验动物消化管的剖检及组织学观察,获得宝贵的组织学资料;通过比较分析,揭示了不同物种组织学特点和进化规律,丰富了比较组织学的研究资料。(2)消化管是与外界相通的开放性管道,易受外界影响而产生自发性病变。Purpose To study the histological differences on digestive tract among rhesus monkeys, beagle dogs, Japanese rabbits, tree shrews, SD rats and KM mice, and to provide a theoretical basis for pathological testing standards of laboratory animals and other scientific studies based on them. Methods 30 rhesus monkeys, 16 beagle dogs, 18 Japanese rabbits, 20 SD rats and 20 KM mice were collected for study. Animal quality and breeding condition complied with the state standard without any infectious disease after quarantine. Tissues of digestive tract were selected after autopsy and euthanasia. Tissues were fixed with 10% neutral formalin, the pathologic slide specimens were prepared by HE staining, immunohistochemical staining and special staining after paraffin imbedding, they were observed and studied under the light microscope. The same and different histological structure characters of them were sum- marized by comparative study. Results In addition to rectal histological structures were equally likely, the following parts are differ- ences ： （ 1 ） The arrangement of the lingualis and the component type of tongue glands, the developed degree of lingual papillae and the number of taste buds. （2） The morphological characters of esophageal mucosa epithelium, the presence or absence of esophageal gland and its composition and the arrangement pattern of muscle layers. （3） Anatomical structures of stomach, the presence or absence of cardiac gland and the histological structures of fundus gland and pylorie gland, the morphological characters of epithelial cells on fundus and AB staining intensity of the cells. （4） The morphological characters of duodenum, jejunum and ileum submucosal. （5） The mu- cosa morphological characters of colon and other special structures of some experimental animals. （6） Inflammation was a common spontaneous lesions of digestive tract. Conclusions （ 1 ） To got some valuable digestive tract histological dates and different histology characters with experimental animal

关 键 词：实验动物 恒河猴 比格犬 树鼩 兔 大鼠 小鼠 消化管 比较组织学 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学]