高脂饲养诱导胰岛素抵抗大鼠脂肪组织C反应蛋白及脂联素的表达  

The expression of C-reactive protein and adiponectin in adipose tissues of insulin resistant rats induced by high fat diet

作　　者：袁国跃[1] 杨玲[1] 贾珏[1] 赵江波[1] 王东[1] 董嗣婧[1] 马勤耘[2] 陈名道[2] 

机构地区：[1]江苏大学附属医院内分泌代谢科,镇江212001 [2]上海交通大学医学院附属瑞金医院,上海市内分泌代谢病临床医学中心,上海市内分泌代谢病研究所

出　　处：《中国糖尿病杂志》2013年第11期1025-1028,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81370965);江苏省卫生厅面上科技项目(H201247);江苏省自然科学基金(BK2009208);镇江市社会发展项目(SH2013027,SH2013035,SH2012027);镇江市医学重点人才项目(2010)

摘　　要：目的探讨C-RP、APN在IR大鼠各组织的表达情况。方法19只SD大鼠,随机分为对照组(NC,n=9)与高脂组(HF,n=10),饲养20周后,行OGTT和胰岛素释放试验(IRT),采用实时荧光定量PCR(RT-PCR)及Northern blot法检测大鼠肝脏、脂肪组织C-RP及APN的表达。结果与NC组比较,HF组血糖、胰岛素水平增加,表明已处于IR状态。Northern blot检测显示,HF组脂肪组织中APN的表达较NC组下降(P<0.01)。RT-PCR显示,HF组肝脏组织C-RP较NC组上升约62%(P<0.01),脂肪组织中C-RP的表达较NC组上升约88%(P<0.01)。结论高脂饲养诱导的IR大鼠,肝脏、脂肪组织C-RP表达增加,而脂肪组织APN表达下降。Objective To explore the expression change of C-reactive protein （C-RP） and adiponectin （APN） in different tissues of insulin resistant rats induced by high fat diet.Methods Nineteen male SD rats were randomized into control group（NC,n=9） and high fat-fed group （HF,n=10）.20 weeks later,OGTT and insulin releasing test（IRT） were detected in all groups.The expression of C-RP and APN mRNA in liver and adipose tissue were detected by real time PCR and northern blot analysis respectively.Results Compared with NC group,plasma glucose and insulin of HF group were increased remarkably,which indicated that the high fat-fed rats were in the state of insulin resistance.Compared with NC group,northern bolt analysis showed that the expression of APN mRNA in adipose tissue of HF group was decreased by 64％（P＜0.01）.Real time PCR results showed that the expression of C-RP mRNA in liver and adipose tissues in HF group was higher than in NC group with 62％ and 88％ increased（P＜0.01）.Conclusion The expression of C-RP in liver and adipose tissues of insulin resistant rats induced by high fat diet is increased.However the expression of APN mRNA in adipose tissue is decreased.

关 键 词：胰岛素抵抗 高脂饲养 C反应蛋白 脂联素 脂肪因子 大鼠 

分 类 号：R587.1[医药卫生—内分泌]