云南美登木内生真菌Alternariasp．Ly81中的混合萜类化合物的研究  

Mixed Terpenoids from an Endophyte Alternaria sp.Ly81 Cultivated on Maytenus hookeri

作　　者：袁琳[1] 黄文忠[1] 赵沛基[2] 

机构地区：[1]昆明学院化学科学与技术系昆明,650204 [2]中国科学院昆明植物研究所,昆明650204

出　　处：《中国药学杂志》2013年第21期1823-1826,共4页Chinese Pharmaceutical Journal

基　　金：云南省应用基础面上项目(2009ZC139M);国家自然科学基金资助项目(31260073)

摘　　要：目的研究云南美登木内生真菌Alternaria sp.Ly81的次生代谢产物,并对所得的单体化合物进行抗肿瘤活性筛选。方法采用RP-18,Sephadex LH-20,硅胶等材料进行色谱柱色谱分离化合物,用现代波谱技术鉴定化合物的结构,用MTT法测试单体化合物活性。结果从4 L固体发酵提取物中分离得到9个混合萜类化合物,分别鉴定为tricycloalternarene 2a(1)、tricycloalternarene 1a(2)、tricycloalternarene 1b(3)、tricycloalternarene 2b(4)、tricycloalternarene 3b(5)、tricycloalternarene 4b(6)、tricycloalternarene 5b(7)、tricycloalternarene-H(8)和tricycloalternarene F(9)。结论化合物1～9均为首次从该菌株中分离得到,且首次用Mosher酯反应确定了化合物1的绝对构型,活性筛选结果显示化合物3对Lewis肺癌细胞3LL有强抑制活性。OBJECTIVE To study the secondary metabolites and their cytotoxic activities of an endophytic fungus Alternaria sp. Ly81 cultivated on Maytenus hookeri. METHODS Column chromatography by RP-18, Sephadex LH -20 and silica gel was used to isolate and purify the chemical constituents, and their structures were identified by spectroscopic analysis. Cytotoxic activities were e- valuated by Mq＇T method. RESULTS Nine compounds were isolated and identified as tricycloaheruarene 2a（ 1 ）, tricycloahernarene 1 a （ 2 ）, tricycloalternarene 1 b （ 3 ） , tricycloahernarene 2 b （4）, tricycloalternarene 3 b （ 5 ）, tricycloahernarene 4 b （ 6 ）, tricycloaherna- rene 5b（7）, tricycloalternarene-H（8） and tricycloaheruarene F（9）. CONCLUSION Compounds 1 -9 were isolated from the Alter- Maria sp. Ly81 for the first time. The absolute configuration of compound 1 is elucidated by Mosher＇s method. Compound 3 shows strong inhibitory activity against Lewis lung carcinoma cells 3LL.

关 键 词：云南美登木 内生真菌 ALTERNARIA sp. 混合萜 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]